isoleucine

製品コードS3752 バッチS375201

印刷

化学情報

Chemical Structure Synonyms Ile Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

C6H13NO2
分子量 131.17 CAS No. 73-32-5
Solubility (25°C)* 体外 Water 26 mg/mL (198.21 mM)
DMSO Insoluble
 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 Isoleucine (Ile) is a non-polar, uncharged (at physiological pH), aliphatic amino acid that is used in the biosynthesis of proteins.
in vitro Isoleucine plays a major role in enhancing glucose consumption and utilization by up-regulating intestinal and muscular glucose transporters[1]. L-isoleucine could induce β-defensins’expressions in porcine intestinal epithelial cells[2].
in vivo Isoleucine is beneficial for mammary health, milk quality and embryo growth, enhances intestinal development, intestinal amino acid transportation and mucin production and participates in up-regulating innate and adaptive immune responses. Studies in mice have indicated that diet-induced obesity mice in an isoleucine treatment (final concentration of 2.5% isoleucine in drinking water) had almost 6% lower body weight gain and 49% less epididymal white adipose tissue mass compared with the control treatment, with higher levels of hepatic protein. Its deprivation induces fat mass loss in mice. Isoleucine could stimulate glucose uptake and participated in plasma glucose uptake in the rat[1].

プロトコル(参考用のみ)

細胞アッセイ 細胞株 IPEC-J2 cells
濃度 0, 25, 50, 100, 250 and 500 μg/mL
反応時間 12 h
実験の流れ After cells are grown to 80 % confluence, they are starved for 12 h in serum and antibiotic-free DMEM/F12 medium. Following starvation, the cells are cultured for 12 h in the presence of 0, 25, 50, 100, 250 and 500 μg/mL L-isoleucine or 0, 25, 50, 100, 250 and 500 μmol/mL zinc sulfate that were used to increase the concentration of Zn2+ in the medium. Then, the cells are collected to examine the mRNA and protein expressions of pBD-1, 2 and 3, respectively.

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。