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Synonyms | angelicin | Storage (From the date of receipt) |
3 years -20°C powder 1 years -80°C in solvent |
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化学式 | C11H6O3 |
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分子量 | 186.16 | CAS No. | 523-50-2 | |
Solubility (25°C)* | 体外 | DMSO | 14 mg/mL (75.2 mM) | |
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. |
製品説明 | Isopsoralen (Angelicin), also known as angelicin, is a constituent of roots and leaves of angelica with anti-inflammatory activity and regulates LPS-induced inflammation via inhibiting MAPK/NF-κB pathways. It also shows antiviral activity against gammaherpesviruses. |
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in vitro | Isopsoralen treatment stimulates the accumulation of cartilage nodules in a dose-dependent manner. Isopsoralen enhances the expressions of chondrogenic marker genes such as collagen II, collagen X, OCN, Smad4 and Sox9 in a time-dependent manner. Furthermore, isopsoralen induces the activation of extracellular signal-regulated kinase (ERK) and p38 MAP kinase, but not that of c-jun N-terminal kinase (JNK). Isopsoralen significantly enhances the protein expression of BMP-2 in a time-dependent manner and mediates a chondromodulating effect by BMP-2 or MAPK signaling pathways[2]. |
in vivo | Isopsoralen has significant osteoprotective effect in female and male mice with sex hormone deprivation. After administration of isopsoralen for 8 weeks, Bone strength increases and trabecular bone microstructure improves[1]. After intravenous administration to wistar rats, the elimination half-lives of isopsoralen is 5.35 h. The area under the curves of the tissues for isopsoralen decrease in the following order: kidney > lung > liver > heart > spleen > brain. After oral administration to Wistar rats, the elimination half-lives of isopsoralen is 5.56 h, and its bioavailability is 70.35%[3]. |
細胞アッセイ | 細胞株 | ATDC5 cells |
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濃度 | 0-1 μM | |
反応時間 | 24 h | |
実験の流れ | ATDC5 cells are seeded in 96-well plate at a density of 5×103 cells/well and incubated overnight in media supplemented with 10% FBS followed by treatment with various concentrations of isopsoralen. After 24h of incubation, cells are washed with phosphate-buffered saline (PBS) and then treated with media containing 100 μg/ml MTT for 2 h at 37℃. The cells are then washed with PBS, and dissolved in 200 μl of DMSO. The resulting solubilized purple formazan is quantified by measuring the absorbance at a wavelength of 540 nm using a spectrophotometer. |
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動物実験 | 動物モデル | ICR mice |
投薬量 | 10 mg/kg and 20 mg/kg | |
投与方法 | intragastrically |
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長期の保管のために-20°Cの下で製品を保ってください。
人間や獣医の診断であるか治療的な使用のためにでない。
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