IWR-1-endo

製品コードS7086 バッチS708604

印刷

化学情報

Synonyms

endo-IWR 1, IWR-1

Storage
(From the date of receipt)

3 years -20°C powder
1 years -80°C in solvent

化学式

C₂₅H₁₉N₃O₃

分子量

409.44

CAS No.

1127442-82-3

Solubility (25°C)*

体外

DMSO

82 mg/mL (200.27 mM)

Water

Insoluble

Ethanol

Insoluble

体内（毎回新しく調製した物を用意してください）

Clear solution	5%DMSO 1 Corn oil	1.5mg/ml	Taking the 1 mL working solution as an example, add 50 μL of 30 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O	4.1mg/ml	Taking the 1 mL working solution as an example, add 50 μL of 82 mg/ml clarified DMSO stock solution to 400 μL PEG300, mix evenly to clarify it; add 50 μL Tween80 to the above system, mix evenly to clarify it; then continue to add 500 μL ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	IWR-1-endo (endo-IWR 1, IWR-1) is a Wnt pathway inhibitor with IC50 of 180 nM in L-cells expressing Wnt3A, induces Axin2 protein levels and promotes β-catenin phosphorylation by stabilizing Axin-scaffolded destruction complexes.
in vitro	Although both IWR-1 and XAV939 act as reversible Wnt pathway inhibitors and exhibit similar pharmacological effects both in vitro and in vivo, IWR-1 exerts its effect via interaction with Axin, while XAV939 binds TNKS directly. ^[1]
in vivo	IWR-1 is a β‐catenin inhibitor.^[3]

プロトコル(参考用のみ)

細胞アッセイ	細胞株	Pig EpiSCs
	濃度	2.5 μM
	反応時間	2 days
	実験の流れ	For neural induction, the 3i/LAF culture medium was replaced with neural induction medium I (2.5 μM IWR-1-endo, 5 μM SB431542, and 10 ng/mL FGF2 in BM) on day 2 after pgEpiSC passage.
動物実験	動物モデル	BALB/c nude mice
	投薬量	160 μg/kg
	投与方法

参考

カスタマーフィードバック

: Data from [Data independently produced by , , Diabetes, 2018, 67(4):554-568]

: Data from [Data independently produced by , , Biochim Biophys Acta Mol Cell Biol Lipids, 2018, 1863(8):834-843]

: Data from [Data independently produced by , , Am J Cancer Res, 2015, 5(10):3123-3134.]

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Derivation of human primordial germ cell-like cells in an embryonic-like culture [ Nat Commun, 2024, 15(1):167]	PubMed: 38167821
Autophagy is essential for human myelopoiesis [ Stem Cell Reports, 2024, 19(2):196-210]	PubMed: 38215759
Inhibitor of Wnt receptor 1 suppresses the effects of Wnt1, Wnt3a and β‑catenin on the proliferation and migration of C6 GSCs induced by low‑dose radiation [ Oncol Rep, 2024, 51(2)22]	PubMed: 38099414
CXXC finger protein 1 -CFP1) bridges the reshaping of genomic H3K4me3 signature to the advancement of lung adenocarcinoma [ Signal Transduct Target Ther, 2023, 8(1):369]	PubMed: 37735441
CXXC finger protein 1 (CFP1) bridges the reshaping of genomic H3K4me3 signature to the advancement of lung adenocarcinoma [ Signal Transduct Target Ther, 2023, 8(1):369]	PubMed: 37735441
A live-cell image-based machine learning strategy for reducing variability in PSC differentiation systems [ Cell Discov, 2023, 9(1):53]	PubMed: 37280224
Direct 3D-Bioprinting of hiPSC-Derived Cardiomyocytes to Generate Functional Cardiac Tissues [ Adv Mater, 2023, 35(52):e2305911]	PubMed: 37655652
Long-term in vivo chimeric cells tracking in non-human primate [ Protein Cell, 2023, pwad049]	PubMed: 37758041
Inhibition of Wnt activity improves peri-implantation development of somatic cell nuclear transfer embryos [ Natl Sci Rev, 2023, 10(9):nwad173]	PubMed: 37593113
Inhibition of Wnt activity improves peri-implantation development of somatic cell nuclear transfer embryos [ Natl Sci Rev, 2023, 10(9):nwad173]	PubMed: 37593113

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。