JSH-23

製品コードS7351 バッチS735101

印刷

化学情報

Chemical Structure Synonyms N/A Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

C16H20N2
分子量 240.34 CAS No. 749886-87-1
Solubility (25°C)* 体外 DMSO 48 mg/mL (199.71 mM)
Ethanol 20 mg/mL (83.21 mM)
Water Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 JSH-23 is an inhibitor of NF-κB transcriptional activity with IC50 of 7.1 μM in RAW 264.7 cell line.
in vitro JSH-23 inhibits LPS-induced nuclear translocation of NF-κB p65 without affecting IκBα degradation. JSH-23 inhibits LPS-induced apoptotic chromatin condensation, while does not show significant cytotoxic effects on the RAW 264.7 cells at <100 μM. [1] JSH-23 also decreases NO production and neuronal migration in LPS activated cultures primary cultures from developing mouse cerebellum. [2] Moreover, JSH-23 augments cisplatin cytotoxicity in ovarian cancer cells with CI values ranging from 0.35 to 0.85. [3]
in vivo JSH-23 (3 mg/kg) significantly reverses the nerve conduction and nerve blood flow deficits by decreasing neuroinflammation and improving antioxidant defence in diabetic rats. [4]

プロトコル(参考用のみ)

キナーゼアッセイ Measurement of NF-κB transcriptional activity
Macrophages RAW 264.7 transfected stably with reporter plasmid of pNF-κB-SEAP-NPT are treated with 1 μg/ml LPS and/or sample for 16 hours. As the reporter, SEAP activity in the cell-free culture media is measured as followed. Single cell-derived stable transfectants are plated in 5 ml of T-25 flask, and the media is decanted 24 h later. At this time, cells are washed twice with phosphate-buffered saline, and incubations are initiated by addition of new media. Chemicals are added to the culture medium after 24 h of incubations. Aliquots (25 ml) of medium from a control or chemical-treated cultures are taken at 0, 3, 20, 24, 48, and 72 h, heated at 65°C for 5 min to eliminate the alkaline phosphatase activity, and used immediately or stored at -20°C. Mixtures consisting of dilution buffer (25 ml), assay buffer (97 ml), culture media (25 ml), and 4-methylumbelliferyl phosphate (MUP, 1 mM, 3 ml) in each well of the 96-well plates are incubated for 60 min in the dark at room temperature. Fluorescence emits the product of the SEAP/MUP is measured at 449 nm using a 96-well plate fluorometer after excitation at 360 nm.
細胞アッセイ 細胞株 RAW 264.7 cells
濃度 ~300 μM
反応時間 24 hours
実験の流れ Macrophages RAW 264.7 are incubated with various concentrations of JSH-23 compound for 24 h. The cells are treated with WST-1 solution and absorbance is measured at 450 nm.
動物実験 動物モデル STZ-induced diabetic rats
投薬量 ~3 mg/kg
投与方法 Oral administration

カスタマーフィードバック

Data from [Data independently produced by , , Cell, 2018, 172(4):841-856]

Data from [Data independently produced by , , Blood, 2015, 125(7): 1098-106 ]

Data from [Data independently produced by , , J Mol Med (Berl), 2018, 96(3-4):301-313]

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Nlrc3 signaling is indispensable for hematopoietic stem cell emergence via Notch signaling in vertebrates [ Nat Commun, 2024, 15(1):226] PubMed: 38172511
Free heme induces neuroinflammation and cognitive impairment by microglial activation via the TLR4/MyD88/NF-κB signaling pathway [ Cell Commun Signal, 2024, 22(1):16] PubMed: 38183122
TP53 gain-of-function mutations promote osimertinib resistance via TNF-α-NF-κB signaling in EGFR-mutated lung cancer [ NPJ Precis Oncol, 2024, 8(1):60] PubMed: 38431700
ERK1/2-CEBPB Axis-Regulated hBD1 Enhances Anti-Tuberculosis Capacity in Alveolar Type II Epithelial Cells [ Int J Mol Sci, 2024, 25(4)2408] PubMed: 38397085
FGF18 alleviates sepsis-induced acute lung injury by inhibiting the NF-κB pathway [ Respir Res, 2024, 25(1):108] PubMed: 38419044
Extensive jejunal injury is repaired by migration and transdifferentiation of ileal enterocytes in zebrafish [ Cell Rep, 2023, 42(7):112660] PubMed: 37342912
Peptidase inhibitor (PI16) impairs bladder cancer metastasis by inhibiting NF-κB activation via disrupting multiple-site ubiquitination of NEMO [ Cell Mol Biol Lett, 2023, 28(1):62] PubMed: 37525118
Peptidase inhibitor (PI16) impairs bladder cancer metastasis by inhibiting NF-κB activation via disrupting multiple-site ubiquitination of NEMO [ Cell Mol Biol Lett, 2023, 28(1):62] PubMed: 37525118
BMP8B Activates Both SMAD2/3 and NF-κB Signals to Inhibit the Differentiation of 3T3-L1 Preadipocytes into Mature Adipocytes [ Nutrients, 2023, 16(1)64] PubMed: 38201894
IL-33/ST2 axis of human amnion fibroblasts participates in inflammatory reactions at parturition [ Mol Med, 2023, 29(1):88] PubMed: 37403020

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人間や獣医の診断であるか治療的な使用のためにでない。

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