L-Mimosine

製品コードS7446 バッチS744601

印刷

化学情報

Chemical Structure Synonyms Leucenol Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

C8H10N2O4
分子量 198.18 CAS No. 500-44-7
Solubility (25°C)* 体外 DMSO 0.01 mg/mL (0.05 mM)
Water Insoluble
Ethanol Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 L-Mimosine (Leucenol) is a non-protein amino acid, and acts as an iron chelator.
in vitro L-Mimosine arrests the cell cycle at the G1 phase in PC-3 cells and at the S phase in LNCaP cells, thus attenuating cell proliferation. [1] L-Mimosine, as a prolyl hydroxylase (PHD) inhibitor, stimulates VEGF production, and increases their proangiogenic capacity in dental pulp-derived cells. [2]
in vivo L-mimosine (50 mg/kg i.p.) substantially upregulates HIF-1alpha expression in the kidneys, and remarkably enhances the natriuretic response to renal perfusion pressure in Sprague-Dawley rats. [3]

プロトコル(参考用のみ)

細胞アッセイ 細胞株 PC-3 and LNCaP cells
濃度 ~800 μM
反応時間 48 hours
実験の流れ Cell proliferation in response to L-mimosine is measured using a [3H]thymidine incorporation assay. In this assay, 1 × 104 cells are cultured in each well of a 12-well plate in RPMI-1640 medium with 10% FCS and different concentrations (0–800 μM) of L-mimosine. After the required incubation periods (24 and 48 h), 0.5 μCi/ml of [3H]thymidine is added to each well of the 12-well plate. The cells are then incubated at 37°C in a humidified 5% CO2 atmosphere for 4 h. Cells are then washed twice with cold phosphate-buffered saline (PBS) and then with cold 5% trichloroacetic acid. Cells are lysed by adding 0.5 ml of 0.5 N NaOH. Then 400 μl of the solubilized cell solution are mixed with 4 ml of scintillation cocktail and counted in a liquid scintillation analyzer . Each sample was tested in quadruplicate.
動物実験 動物モデル Sprague-Dawley rats
投薬量 ~50 mg/kg
投与方法 i.p.

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

DNA nicks induce mutational signatures associated with BRCA1 deficiency [ Nat Commun, 2022, 13(1):4285] PubMed: 35879372
DNA nicks induce mutational signatures associated with BRCA1 deficiency [ Nat Commun, 2022, 13(1):4285] PubMed: 35879372
Identification of amitriptyline HCl, flavin adenine dinucleotide, azacitidine and calcitriol as repurposing drugs for influenza A H5N1 virus-induced lung injury. [ PLoS Pathog, 2020, 16;16(3):e1008341] PubMed: 32176725
CCDC84 Acetylation Oscillation Regulates Centrosome Duplication by Modulating HsSAS-6 Degradation [ Cell Rep, 2019, 29(7):2078-2091.e5] PubMed: 31722219
M-Phase Phosphoprotein 9 regulates ciliogenesis by modulating CP110-CEP97 complex localization at the mother centriole. [ Nat Commun, 2018, 9(1):4511] PubMed: 30375385

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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