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Synonyms | Leucenol | Storage (From the date of receipt) |
3 years -20°C powder 1 years -80°C in solvent |
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化学式 | C8H10N2O4 |
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分子量 | 198.18 | CAS No. | 500-44-7 | |
Solubility (25°C)* | 体外 | 5%TFA | 3.04 mg/mL (15.33 mM) | |
DMSO | 0.01 mg/mL (0.05 mM) | |||
Water | Insoluble | |||
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. |
製品説明 | L-Mimosine (Leucenol) is a non-protein amino acid, and acts as an iron chelator. |
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in vitro | L-Mimosine arrests the cell cycle at the G1 phase in PC-3 cells and at the S phase in LNCaP cells, thus attenuating cell proliferation. [1] L-Mimosine, as a prolyl hydroxylase (PHD) inhibitor, stimulates VEGF production, and increases their proangiogenic capacity in dental pulp-derived cells. [2] |
in vivo | L-mimosine (50 mg/kg i.p.) substantially upregulates HIF-1alpha expression in the kidneys, and remarkably enhances the natriuretic response to renal perfusion pressure in Sprague-Dawley rats. [3] |
細胞アッセイ | 細胞株 | PC-3 and LNCaP cells |
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濃度 | ~800 μM | |
反応時間 | 48 hours | |
実験の流れ | Cell proliferation in response to L-mimosine is measured using a [3H]thymidine incorporation assay. In this assay, 1 × 104 cells are cultured in each well of a 12-well plate in RPMI-1640 medium with 10% FCS and different concentrations (0–800 μM) of L-mimosine. After the required incubation periods (24 and 48 h), 0.5 μCi/ml of [3H]thymidine is added to each well of the 12-well plate. The cells are then incubated at 37°C in a humidified 5% CO2 atmosphere for 4 h. Cells are then washed twice with cold phosphate-buffered saline (PBS) and then with cold 5% trichloroacetic acid. Cells are lysed by adding 0.5 ml of 0.5 N NaOH. Then 400 μl of the solubilized cell solution are mixed with 4 ml of scintillation cocktail and counted in a liquid scintillation analyzer . Each sample was tested in quadruplicate. | |
動物実験 | 動物モデル | Sprague-Dawley rats |
投薬量 | ~50 mg/kg | |
投与方法 | i.p. |
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DNA nicks induce mutational signatures associated with BRCA1 deficiency [ Nat Commun, 2022, 13(1):4285] | PubMed: 35879372 |
DNA nicks induce mutational signatures associated with BRCA1 deficiency [ Nat Commun, 2022, 13(1):4285] | PubMed: 35879372 |
Identification of amitriptyline HCl, flavin adenine dinucleotide, azacitidine and calcitriol as repurposing drugs for influenza A H5N1 virus-induced lung injury. [ PLoS Pathog, 2020, 16;16(3):e1008341] | PubMed: 32176725 |
CCDC84 Acetylation Oscillation Regulates Centrosome Duplication by Modulating HsSAS-6 Degradation [ Cell Rep, 2019, 29(7):2078-2091.e5] | PubMed: 31722219 |
M-Phase Phosphoprotein 9 regulates ciliogenesis by modulating CP110-CEP97 complex localization at the mother centriole. [ Nat Commun, 2018, 9(1):4511] | PubMed: 30375385 |
長期の保管のために-20°Cの下で製品を保ってください。
人間や獣医の診断であるか治療的な使用のためにでない。
各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。