L-Theanine

製品コードS3852 バッチS385201

化学情報

	Synonyms	N/A	Storage (From the date of receipt)	3 years -20°C powder 1 years -80°C in solvent
	化学式	C₇H₁₄N₂O₃
	分子量	174.20	CAS No.	3081-61-6
Solubility (25°C)*	体外	Water	34 mg/mL (195.17 mM)
		DMSO	Insoluble

* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	L-Theanine is a relaxing and nondietary amino acid found pretty much exclusively in teas from Camellia sinensis and is known to promote relaxation without sedation.
in vitro	L-theanine promotes NO production in endothelial cells. L-Theanine mediated eNOS phosphorylation and NO production are PI3K/ERK pathway dependant. Treatment of EA.hy926 cells with L-theanine dose-dependently increases eNOS Ser1177 phosporylation. This increase occurrs at 2-5 min of L-theanine treatment and coincides with NO production. L-theanine confers neuroprotective effects by increasing the expression levels of PLC-beta1 and gamma1, and thereby minimizes apoptosis in the neural cells^[2].
in vivo	L-theanine can enhance innate immune function by regulating the secretion of immune cytokines. Its administration increases the splenic organ index and decreases the contents of ILs-4/6/10 and the ratio of IL-4/IFN-γ in the serum. High-dose L-theanine administration increases the levels of dopamine and 5-hydroxytryptamine in the pituitary and hippocampus, resulting in decrease in corticosterone level in the serum. L-theanine administration decreases the mRNA expressions of PLC isomers in the liver and PLC-γ1 and PLC-δ1 in the spleen. mRNA expressions of PLC-βf1 in the spleen and PLC isomers mRNA in the heart are up-regulated by L-theanine administration. After being absorbed into the body, L-theanine can penetrate the blood-brain barrier and regulate the secretion of neurotransmitters of central nervous system cells and levels of hormones such as corticosterone (CORT) and adrenaline (EPI) in the serum. L-theanine can increase levels of 5-hydroxytryptamine (5-HT) and noradrenaline, and decrease levels of adrenocorticotropic hormone and CORT in the hippocampus and prefrontal cortex of rats^[1]. L-theanine has been implicated in reduction of blood pressure in hypertensive rats^[2]. L-theanine could inhibit the glucose uptake by downregulating the related gene expression in the small intestine of rats. Intestinal gene expression of transporters responding to amino acids absorption was stimulated by L-theanine administration^[3].

プロトコル(参考用のみ)

細胞アッセイ	細胞株	EA.hy926 cells
	濃度	0.01, 0.1, 1, 10 μM
	反応時間	30 min
	実験の流れ	EA.hy926 cells are treated with varying concentration of L-theanine (0.01 μM, 0.1 μM, 1.0 μM, 10 μM) for 30 min and after 24 h of incubation cell viability was determined using CMFDA as described elsewhere.
動物実験	動物モデル	Sprague-Dawley rats
	投薬量	0, 50, 200, and 400 mg/kg BW
	投与方法	intragastric infusion

参考

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。