Leupeptin Hemisulfate

製品コードS7380 バッチS738019

印刷

化学情報

Chemical Structure Synonyms N/A Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

C20H38N6O4.1/2H2SO4
分子量 475.59 CAS No. 103476-89-7
Solubility (25°C)* 体外 DMSO 95 mg/mL warmed with 50ºC water bath (199.75 mM)
Water 95 mg/mL (199.75 mM)
Ethanol 95 mg/mL (199.75 mM)
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 Leupeptin Hemisulfate is a reversible inhibitor of serine and cysteine proteases. It inhibits cathepsin B (Ki = 6 nM), calpain (Ki = 10 nM), trypsin (Ki = 35 nM), plasmin (Ki = 3.4 μM), and kallikrein (Ki = 19 μM), and has no effect against chymotrypsin, elastase, renin, or pepsin.
in vitro

Leupeptin, produced by various species of actinomycetes, strongly inhibit proteolysis. [1]

Leupeptin hemisulfate protects tubulin from endogenous proteolytic activities during the isolation procedure and results in increased tubulin purity. [2]

Leupeptin hemisulfate could restore up to 50% of hepatitis B surface antigen (HBsAg) expression in cell suspension cultures. [3]

プロトコル(参考用のみ)

細胞アッセイ 細胞株 Cell-free assays
濃度 Ki of 6 nM (cathepsin B), 10 nM (calpain), 35 nM (trypsin), 3.4 μM (plasmin), and 19 μM (kallikrein).
反応時間
実験の流れ
動物実験 動物モデル C57BL/6NCrl male mice
投薬量 20 mg/kg
投与方法 i.p.

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長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。