Lycorine

製品コードS3903 バッチS390303

印刷

化学情報

Synonyms

Galanthidine, Amarylline, Narcissine, Licorine, Belamarine

Storage
(From the date of receipt)

3 years -20°C powder
1 years -80°C in solvent

化学式

C₁₆H₁₇NO₄

分子量

287.31

CAS No.

476-28-8

Solubility (25°C)*

体外

DMSO

40 mg/mL (139.22 mM)

Water

Insoluble

Ethanol

Insoluble

体内（毎回新しく調製した物を用意してください）

Clear solution

5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O

2.0mg/ml

Taking the 1 mL working solution as an example, add 50 μL of 40 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	Lycorine (Galanthidine, Amarylline, Narcissine, Licorine, Belamarine) is a toxic crystalline alkaloid found in various Amaryllidaceae species that weakly inhibits acetylcholinesterase (AChE) and ascorbic acid biosynthesis.
in vitro	Lycorine can inhibit protein synthesis in eukaryotic cells and acetylcholinesterase activity. Lycorine could effectively arrest the cell cycle at the G2/M phase and induce apoptosis in HL-60 cells. It is able to block the KM3 cell cycle at G0/G1 phase through the down-regulation of both cyclin D1 and CDK4^[2].
in vivo	Lycorine, when tested in the human leukemia xenograft models, appears to exhibit anti-tumor activity in vivo. It prolongs the mean survival time of SCID leukemia xenograft models. Lycorine could alleviate the infiltration of tumor cells into tissues^[2].

プロトコル(参考用のみ)

細胞アッセイ	細胞株	Caki-1; ACHN; KPK-1 and Renca cell lines
	濃度	0, 0.5, 1, 5, and 10 μM
	反応時間	24, 48, 72, and 96 h
	実験の流れ	The cell lines Caki-1; ACHN; KPK-1 and Renca are diluted in 96-well plates at a density of 1 × 10³ and incubated with medium alone or serial dilutions of lycorine hydrochloride (0, 0.5, 1, 5, and 10 μM). Cell viability is monitored after 24, 48, 72, and 96 h. For each time point, the XTT labeling reagent and an electron coupling reagent is added to the culture. The cells are incubated for 4 h at 37°C and 5% CO2, and absorbance at 450–500 nm, with a reference wavelength at 650 nm, is recorded using a Bio-Rad microplate reader.
動物実験	動物モデル	C57BL/6 mice inoculated with Renca-Luc cells into the sub-renal capsule kidney and tail vein (orthotopic tumor and metastatic lung tumors model)
	投薬量	5 mg/kg
	投与方法	i.p.

参考

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Lycorine weakens tamoxifen resistance of breast cancer via abrogating HAGLR-mediated epigenetic suppression on VGLL4 by DNMT1 [ Kaohsiung J Med Sci, 2023, none]	PubMed: 36606584
A multi-targeting drug design strategy for identifying potent anti-SARS-CoV-2 inhibitors [ Acta Pharmacol Sin, 2021, 1-11]	PubMed: 33907306

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。