MCC950

製品コードS8930 バッチS893002

印刷

化学情報

Chemical Structure Synonyms CP-456773, CRID3 Storage
(From the date of receipt)		 3 years -20°C powder
化学式

C20H24N2O5S
分子量 404.48 CAS No. 210826-40-7
Solubility (25°C)* 体外 DMSO 81 mg/mL (200.25 mM)
Ethanol 7 mg/mL (17.3 mM)
Water Insoluble
体内 （毎回新しく調製した物を用意してください）
Clear solution
5%DMSO 40%PEG300 5%Tween80 50%ddH2O
2.5mg/ml Taking the 1 mL working solution as an example, add 50 μL of 50 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to make it clear; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 MCC950 (CP-456773, CRID3) is a potent and selective inhibitor of NLRP3 (NOD-like receptor (NLR) family, pyrin domain-containing protein 3) with IC50 of 7.5 nM and 8.1 nM in BMDMs and HMDMs, respectively.

生物活性

製品説明 MCC950 (CP-456773, CRID3) is a potent and selective inhibitor of NLRP3 (NOD-like receptor (NLR) family, pyrin domain-containing protein 3) with IC50 of 7.5 nM and 8.1 nM in BMDMs and HMDMs, respectively.
in vitro

MCC950 specifically inhibits NLPR3 inflammasome activation.[1]
in vivo

Inhibition of NLRP3 with MCC950 ameliorates proteinuria, renal histological lesions and podocyte foot process effacement in lupus-prone mice.[1]

プロトコル(参考用のみ)

細胞アッセイ 細胞株 NZM2328 podocyte cell line
濃度 1 µM
反応時間 24 h
実験の流れ

The NZM2328 podocyte cell line was stimulated with a control serum or a serum from diseased NZM2328 mice or serum from diseased NZM2328 mice depleted of IgG, with or without MCC950 or Mito TEMPO for 24 hours. After this incubation, cells were subjected to flow cytometry and Western blot analyses for NLRP3 inflammasome activation, mitochondrial membrane potential and production of reactive oxygen species (ROS) analysis.

(Data sourced from selleck products)
動物実験 動物モデル female NZM2328, a new NZM strain produced by extended backcrosses of NZB/WF1 to NZW mice
投薬量 10mg/kg
投与方法 i.p.

(Data sourced from selleck products)

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

METTL14 contributes to acute lung injury by stabilizing NLRP3 expression in an IGF2BP2-dependent manner [ Cell Death Dis, 2024, 15(1):43] PubMed: 38218935
ChemR23 signaling ameliorates brain injury via inhibiting NLRP3 inflammasome-mediated neuronal pyroptosis in ischemic stroke [ J Transl Med, 2024, 22(1):23] PubMed: 38178174
Bacillus cereus cereolysin O induces pyroptosis in an undecapeptide-dependent manner [ Cell Death Discov, 2024, 10(1):122] PubMed: 38458999
Melatonin alleviates chronic stress-induced hippocampal microglia pyroptosis and subsequent depression-like behaviors by inhibiting Cathepsin B/NLRP3 signaling pathway in rats [ Transl Psychiatry, 2024, 14(1):166] PubMed: 38538614
From inflammation to pyroptosis: Understanding the consequences of cadmium exposure in chicken liver cells [ Ecotoxicol Environ Saf, 2024, 272:116004] PubMed: 38290315
Cooperative induction of CXCL chemokines by inflammatory cytokines and oxidized phospholipids [ Immunology, 2024, 10.1111/imm.13773] PubMed: 38468451
GLUT1 promotes NLRP3 inflammasome activation of airway epithelium in lipopolysaccharide-induced acute lung injury [ Am J Pathol, 2024, S0002-9440(24)00116-0] PubMed: 38548270
NLRP3 inflammasome mediates abnormal epithelial regeneration and distal lung remodeling in silica‑induced lung fibrosis [ Int J Mol Med, 2024, 53(3)25] PubMed: 38240085
Lactobacillus Plantarum Promotes Wound Healing by Inhibiting the NLRP3 Inflammasome and Pyroptosis Activation in Diabetic Foot Wounds [ J Inflamm Res, 2024, 17:1707-1720] PubMed: 38510374
Poly-(ADP-ribose) polymerases inhibition by olaparib attenuates activities of the NLRP3 inflammasome and of NF-κB in THP-1 monocytes [ PLoS One, 2024, 19(2):e0295837] PubMed: 38335214

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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