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Synonyms | N/A | Storage (From the date of receipt) |
3 years -20°C powder 1 years -80°C in solvent |
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化学式 | C9H10O4 |
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分子量 | 182.17 | CAS No. | 3943-74-6 | |
Solubility (25°C)* | 体外 | DMSO | 36 mg/mL (197.61 mM) | |
Ethanol | 36 mg/mL (197.61 mM) | |||
Water | 12 mg/mL (65.87 mM) | |||
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. |
製品説明 | Methyl Vanillate, one of the ingredients in Hovenia dulcis Thunb, activates the Wnt/β-catenin pathway and induces osteoblast differentiation in vitro. |
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in vitro | Methyl vanillate (MV) activates the Wnt/β-catenin pathway and induces osteoblast differentiation in vitro. It increases the expression of differentiation markers RUNX2, BMP2, ALP, and OCN in a dose-dependent manner. MV decreases and increases the expression of RANKL and OPG, respectively. Methyl vanillate dose-dependently elevates ALP activity in calvarial osteoblasts[1]. |
in vivo | Methyl vanillate rescues trabecular or cortical femoral bone loss in the ovariectomized mice without inducing any significant weight changes or abnormality in liver tissue when administrated orally[1]. |
細胞アッセイ | 細胞株 | Calvarial osteoblasts |
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濃度 | 10 and 20 μM | |
反応時間 | 24 h | |
実験の流れ | Calvarial osteoblasts are seeded onto a coverslip in 12-well plates at a density of 3×104 cells/well. After 24 h, HDT extract or MV is added for another 24 h and the cells are subsequently fixed with 4% PFA for 10 min. Cells are permeabilized with 0.2% Triton X-100 for 15 min and incubated for 30 min with 5% bovine serum albumin (BSA) blocking solution. The cells are incubated with the primary antibody (β-catenin) overnight at 4°C and then washed with PBS three times. Cells are then incubated with Alexa Fluor 502-conjugated IgG secondary antibody is incubated for 1 h, followed by incubation with DAPI for 5 min. The cells are mounted in Gel/Mount media. The fluorescence signal is captured using confocal microscopy. | |
動物実験 | 動物モデル | ICR mice |
投薬量 | 25 and 100 mg/kg | |
投与方法 | oral |
Establishment and characterization of immortalized sweat gland myoepithelial cells [ Sci Rep, 2022, 12(1):7] | PubMed: 34997030 |
長期の保管のために-20°Cの下で製品を保ってください。
人間や獣医の診断であるか治療的な使用のためにでない。
各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。