Methylene Blue

製品コードS4535 バッチS453501

印刷

化学情報

Chemical Structure Synonyms Basic Blue 9, Tetramethylthionine chloride, methylthioninium chloride, CI-52015 Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

C16H18ClN3S
分子量 319.85 CAS No. 61-73-4
Solubility (25°C)* 体外 DMSO 63 mg/mL warmed with 50ºC water bath (196.96 mM)
Water 63 mg/mL warmed with 50ºC water bath (196.96 mM)
Ethanol 10 mg/mL warmed with 50ºC water bath (31.26 mM)
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 Methylene Blue (Basic Blue 9, Tetramethylthionine chloride, methylthioninium chloride, CI-52015) is used as a dye in chromoendoscopy. It Inhibits tau filament formation with IC50 of 1.9μM. Also it inhibits soluble guanylyl cyclase.
in vitro MB may inhibit the action of nitric oxide synthase itself and could also inactivate nitric oxide directly, possibly through the generation of superoxide anions. In addition to interfering with the nitric oxide–cyclic guanosine monophosphate pathway, MB has also been shown to directly activate calcium-dependent potassium channels and to enhance release of noradrenaline from intracellular stores[1].
in vivo Methylene Blue acts by inhibiting guanylate cyclase, thus decreasing C-GMP and vascular smooth muscle relaxation. When injected intravenously, MB selectively accumulates in parathyroid glands, thereby facilitating the identification of these structures during surgery. Pharmacokinetic studies in rodents revealed that intravascular administration of MB leads to a rapid and extensive accumulation of this drug in the central nervous system (CNS)[1].

プロトコル(参考用のみ)

細胞アッセイ 細胞株 Immature neuroblasts
濃度 10 μM
反応時間 2 h
実験の流れ

single cell culture model where immature neuroblasts, isolated from the newborn rat subventricular zone, differentiate into γ-aminobutyric acid–mediated neurons are usd for investigations. Cultures are exposed at the sixth day in vitro to MB for 2 h and then we assess the survival and dendritic arbor architecture of these cells 48 h after this treatment paradigm.
動物実験 動物モデル Sprague- Dawley male rats
投薬量 5 and 50 mg/kg
投与方法 intravascular injection

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Dissecting peri-implantation development using cultured human embryos and embryo-like assembloids [ Cell Res, 2023, 10.1038/s41422-023-00846-8] PubMed: 37460804
Reversal of Infected Host Gene Expression Identifies Repurposed Drug Candidates for COVID-19 [ bioRxiv, 2020, 2020/9/20.4.7.30734] PubMed: 32511305

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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