MHY1485

製品コードS7811 バッチS781102

印刷

化学情報

Chemical Structure Synonyms N/A Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

C17H21N7O4
分子量 387.39 CAS No. 326914-06-1
Solubility (25°C)* 体外 DMSO 3.75 mg/mL (9.68 mM)
Water Insoluble
Ethanol Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 MHY1485 is a potent, and cell-permeable mTOR activator, and also potently inhibits autophagy.
in vitro

MHY1485 suppresses the basal autophagic flux. MHY1485 causes the accumulation of LC3II and enlargement of the autophagosomes in a dose- and time- dependent manner.[1]

MHY1485 increases phospho-mTOR levels and phosphorylation of downstream S6K1 and rpS6 proteins without affecting total mTOR content, total S6K1 and rpS6 levels. Short-term treatment of ovaries with MHY1485 followed by allo-transplantation promoted secondary follicle growth. Treatment with MHY1485 and subsequent grafting allowed the derivation of mature oocytes and healthy offspring.[2]
in vivo

MHY1485 is a potent, and cell-permeable mTORC1 agonist.

プロトコル(参考用のみ)

キナーゼアッセイ Activation of mTOR
Western blot analysis is performed to detect the change of total protein level and levels of phosphorylated forms of mTOR and 4E-BP1 reflecting the activity of mTOR. Ac2F cells are treated with MHY1485 of different concentrations and rapamycin 5 mM as a positive control for 1 h. Cells are washed with cold PBS and harvested. Cell lysates are prepared using RIPA buffer (50 mM Tris-HCl, 150 mM NaCl, 1% NP-40, 1 mM DTT, 0.1 mM NaF, 1 mM PMSF, 1 mg/mL pepstatin, 1 mg/mL leupeptin, and 1 mg/mL aprotinin). Protein concentration is determined by the bicinchoninic acid (BCA) method. Equal amounts of protein are separated on 10-12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) gels. The gels are subsequently transferred onto a polyvinylidene difluoride membrane by electroblotting for 2 h at 60-75 V. The membranes are blocked in a 5% nonfat milk solution in Tris-buffered saline (TBS) with 0.5% Tween-20, and incubated with primary antibodies. Pre-stained protein markers are used for molecular-weight determination.
細胞アッセイ 細胞株 TU177 cells
濃度 10 µM
反応時間 24 h
実験の流れ

Cells were treated with indicated concentration of drug for 24 h.
動物実験 動物モデル C57BL/6 mice
投薬量 5 mg/kg
投与方法 i.p.

カスタマーフィードバック

Data from [Data independently produced by , , FASEB J, 2018, 32(1):512-528]

Data from [Data independently produced by , , Int J Biol Sci, 2017, 13(11):1398-1408]

Data from [Data independently produced by , , Biochem J, 2017, 474(16):2733-2747]

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Gestational exposure to PM2.5 disrupts fetal development by suppressing placental trophoblast syncytialization via progranulin/mTOR signaling [ Sci Total Environ, 2024, 921:171101] PubMed: 38387595
Metformin improves memory via AMPK/mTOR-dependent route in a rat model of Alzheimer's disease [ Iran J Basic Med Sci, 2024, 27(3):360-365.] PubMed: 38333746
MHY1485 potentiates immunogenic cell death induction and anti-cancer immunity following irradiation [ J Radiat Res, 2024, rrad107] PubMed: 38330507
Antitumor activity of afatinib in EGFR T790M-negative human oral cancer therapeutically targets mTOR/Mcl-1 signaling axis [ Research Square, 2024, 10.21203/rs.3.rs-3872267/v1] PubMed: none
Cold exposure protects against medial arterial calcification development via autophagy [ J Nanobiotechnology, 2023, 21(1):226] PubMed: 37461031
Butyrate dictates ferroptosis sensitivity through FFAR2-mTOR signaling [ Cell Death Dis, 2023, 14(4):292] PubMed: 37185889
A novel gene REPTOR2 activates the autophagic degradation of wing disc in pea aphid [ Elife, 2023, 12e83023] PubMed: 36943031
A novel gene REPTOR2 activates the autophagic degradation of wing disc in pea aphid [ Elife, 2023, 12e83023] PubMed: 36943031
Propionate promotes gluconeogenesis by regulating mechanistic target of rapamycin (mTOR) pathway in calf hepatocytes [ Anim Nutr, 2023, 10.1016/j.aninu.2023.07.001] PubMed: 37841648
A unique poly(A) tail profile uncovers the stability and translational activation of TOP transcripts during neuronal differentiation [ iScience, 2023, 26(9):107511] PubMed: 37636056

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。