Mitomycin C

製品コードS8146 バッチS814605

印刷

化学情報

Chemical Structure Synonyms Ametycine Storage
(From the date of receipt)		 3 years -20°C(in the dark) powder
化学式

C15H18N4O5
分子量 334.37 CAS No. 50-07-7
Solubility (25°C)* 体外 DMSO 67 mg/mL (200.37 mM)
Water Insoluble
Ethanol Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 Mitomycin C (Ametycine) is an antineoplastic antibiotic by inhibiting DNA synthesis, used to treat different cancers. Mitomycin C induces apoptosis in a caspases-dependent and Fas/CD95-independent manner.This product is a hazardous chemical (acute toxicity/flammable/skin corrosive). Please use it while wearing a protective face mask, gloves, and clothing.
in vitro

Mitomycin C, by inducing DNA interstrand crosslinks, physically blocks DNA replication, recombination, and RNA transcription. [1] Mitomycin C potentiates TRAIL-induced apoptosis in HCT116 (p53-/-) colon cancer cells and sensitizes TRAIL-resistant colon cancer cells HT-29 to the cytokine by through JNK-independent upregulation of death receptors. [2] In different human cancer cell lines, such OVCAR-5 (ovary), HT-29 (colon), SK-N-MC (neuroblastoma), HEP-2 (liver), COLO-205 (colon), NIH-OVCAR-3 (ovary) and A-549 (lung) cells, Mitomycin C shows cytotoxic activity. [3]
in vivo

As the clinical first choice in superficial bladder tumors, in a rat bladder tumor model, Mitomycin C (400 μM) significantly prevents intravesical tumor growth. [4]

プロトコル(参考用のみ)

細胞アッセイ 細胞株 HCT116 cells
濃度 5 µM
反応時間 24 h
実験の流れ

Cells were pre-treated with 5 µM MMC for 24 h and then co-treated with TRAIL (25 ng/mL).
動物実験 動物モデル Rat bladder tumor model
投薬量 400 μM
投与方法 Intravesical treatment

カスタマーフィードバック

Data from [Data independently produced by , , Cancer Sci, 2018, 109(11):3450-3460]

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人間や獣医の診断であるか治療的な使用のためにでない。

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