ML347

製品コードS7148 バッチS714801

印刷

化学情報

Chemical Structure Synonyms LDN-193719 Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

C22 H16 N4 O 
分子量 352.39 CAS No. 1062368-49-3
Solubility (25°C)* 体外 DMSO 10 mg/mL (28.37 mM)
Water Insoluble
Ethanol Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 ML347 (LDN-193719) is a selective BMP receptor inhibitor with IC50 of 32 nM for ALK2, >300-fold selectivity over ALK3. Also inhibits ALK1 activity with IC50 of 46 nM.
in vitro ML347 also shows potency in BMP-responsive C2C12BRA cells with IC50 of 15 nM, and designated an MLPCN probe. ML347 displays high plasma protein binding in human, rat and mouse plasma. [1]

プロトコル(参考用のみ)

キナーゼアッセイ Kinase assays
In brief, compounds are tested at 10 concentrations by threefold serial dilutions starting at 100 mM. In vitro kinase reactions are carried out in the presence of 10 μM (33P)ATP. Eleven human kinases tested are the BMP type-I receptors ALK-1/ACVRL1, ALK2/ACRV1, ALK3/BMPR1A and ALK6/BMPR1B, the TGFb type-I receptors ALK4/ACVR1B and ALK5/TGFbR1, the BMP type-II receptor (BMPR2), the TGFb type-II receptor (TGFbR2), VEGF type-II receptor (KDR/VEGFR2), AMP-activated protein kinase (AMPK-A1/B1/G1) and the human platelet-derived growth factor receptor-β (PDGFRβ).

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

A pendulum of induction between the epiblast and extra-embryonic endoderm supports post-implantation progression [ Development, 2022, 149(20)dev192310] PubMed: 35993866
Activins as Dual Specificity TGF-β Family Molecules: SMAD-Activation via Activin- and BMP-Type 1 Receptors [ Biomolecules, 2020, 10(4)E519] PubMed: 32235336
Activins as Dual Specificity TGF-β Family Molecules: SMAD-Activation via Activin- and BMP-Type 1 Receptors. [ Biomolecules, 2020, 29;10(4) pii: E519] PubMed: 32235336
TGFβ1 Induces Axonal Outgrowth via ALK5/PKA/SMURF1-Mediated Degradation of RhoA and Stabilization of PAR6 [ eNeuro, 2020, 7(5):ENEURO.0104-20.2020] PubMed: 32887692
Developing a Novel and Convenient Model for Investigating Sweat Gland Morphogenesis from Epidermal Stem Cells [ Stem Cells Int, 2019, 2019:4254759] PubMed: 30863451
Developing a Novel and Convenient Model for Investigating Sweat Gland Morphogenesis from Epidermal Stem Cells [Tian Hu, et al. Stem Cells International, 2019, 10.1155/2019/4254759]

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。