ML390

製品コードS8382 バッチS838201

化学情報

	Synonyms	N/A	Storage (From the date of receipt)	3 years -20°C powder 1 years -80°C in solvent
	化学式	C₂₁H₂₁F₃N₂O₃
	分子量	406.4	CAS No.	2029049-79-2
Solubility (25°C)*	体外	DMSO	81 mg/mL (199.31 mM)
		Ethanol	60 mg/mL (147.63 mM)
		Water	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	ML390 is a human DHODH inhibitor that induces differentiation in acute myeloid leukemia.
in vitro	ML390 was active with an ED50 (effective concentration triggering 50% of its maximal differentiation activity) of 2 μM in murine and human AML cell lines, offering insight into the mechanism of overcoming differentiation arrest. DHODH inhibitors demonstrated effective at prolonging survival in animal models of leukemia^[1].

プロトコル(参考用のみ)

細胞アッセイ	細胞株	Lys-GFP-ER-HoxA9 cells
	濃度	10 μM
	反応時間	48 h
	実験の流れ	Lys-GFP-ER-HoxA9 cells were treated in culture with 10 μM ML390 for 48 hr. The cells were washed three times in normal saline, and metabolites were extracted into 80% ice-cold methanol. Metabolites were analyzed. Negative ionization mode analyses of polar metabolites were acquired using an LC-MS system comprising an Acquity UPLC System (Waters) and a 5500 QTrap triple quadrupole mass spectrometer (AB SCIEX). Samples for negative ion mode analyses of polar metabolites were achieved using the HILIC (hydrophilic interaction chromatography) method under basic conditions as described previously, and MS data were acquired over m/z 70-750. MS data were processed using MultiQuant.

参考

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Identification of dihydroorotate dehydrogenase inhibitor, vidofludimus, as a potent and novel inhibitor for influenza virus [ J Med Virol, 2024, 96(1):e29372]	PubMed: 38235544
Identification of clinical candidates against West Nile Virus by activity screening in vitro and effect evaluation in vivo [ J Med Virol, 2022, 10.1002/jmv.27891]	PubMed: 35644833

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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