Necrosulfonamide

製品コードS8251 バッチS825102

印刷

化学情報

Chemical Structure Synonyms N/A Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

C18H15N5O6S2
分子量 461.47 CAS No. 1360614-48-7
Solubility (25°C)* 体外 DMSO 92 mg/mL (199.36 mM)
Water Insoluble
Ethanol Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 Necrosulfonamide is a very specific and potent necrosis inhibitor and blocks mixed lineage kinase domain-like protein (MLKL).
in vitro

Necrosulfonamide inhibits MLKL-mediated Necrosis by blocking its N-terminal CC domain function. It blocks necrosis downstream of RIP3 activation. Necrosulfonamide has no effect on apoptosis induced by TNF-α plus Smac mimetic in non-RIP3-expressing Panc-1 cells, even at 5 μM concentration. The reason for the species specificity of necrosulfonamide is that the cysteine at residue 86 in human MLKL that is covalently modified by necrosulfonamide is replaced by a tryptophan residue in mouse MLKL(mixed lineage kinase domain-like protein)[2].
in vivo

Necrosulfonamide (NSA) is a small molecule that specifically inhibits necroptosis by targeting MLKL, the terminal executioner of necroptosis.

プロトコル(参考用のみ)

細胞アッセイ 細胞株 HT-29 cells
濃度 1 μM
反応時間 8 or 12 hrs
実験の流れ

Necrosis inhibitors induce diverse effects on MLKL phosphorylation. HT-29 cells are treated with T/S/Z with or without necrosis inhibitors for 12 hr or 8 hr. The number of dead cells is determined by measuring released protease activity in culture medium. The whole-cell extracts are prepared and analyzed by western blotting. The final concentrations of 10 μM necrostatin-1 or 1 μM necrosulfonamide are used to block necrosis.
動物実験 動物モデル Male Wistar rats
投薬量 1.65 mg/kg
投与方法 i.p.

カスタマーフィードバック

Data from [Data independently produced by , , Biochim Biophys Acta, 2018, 1865(3):522-531]

Data from [Data independently produced by , , Biochem Biophys Res Commun, 2018, 503(3):1550-1556]

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

NR4A1 depletion inhibits colorectal cancer progression by promoting necroptosis via the RIG-I-like receptor pathway [ Cancer Lett, 2024, 585:216693] PubMed: 38301909
The cell fate regulator DACH1 modulates ferroptosis through affecting P53/SLC25A37 signaling in fibrotic disease [ Hepatol Commun, 2024, 8(3)e0396] PubMed: 38437058
Gasdermin D regulates soluble fms-like tyrosine kinase 1 release in macrophages [ Reprod Biol, 2024, 24(1):100857] PubMed: 38295720
Fatostatin induces ferroptosis through inhibition of the AKT/mTORC1/GPX4 signaling pathway in glioblastoma [ Cell Death Dis, 2023, 14(3):211] PubMed: 36966152
Hinokitiol-iron complex is a ferroptosis inducer to inhibit triple-negative breast tumor growth [ Cell Biosci, 2023, 13(1):87] PubMed: 37179385
NF-κB Inhibitor Myrislignan Induces Ferroptosis of Glioblastoma Cells via Regulating Epithelial-Mesenchymal Transformation in a Slug-Dependent Manner [ Oxid Med Cell Longev, 2023, 2023:7098313] PubMed: 36699318
Second generation androgen receptor antagonist, TQB3720 abrogates prostate cancer growth via AR/GPX4 axis activated ferroptosis [ Front Pharmacol, 2023, 14:1110146] PubMed: 36744249
Flanking N- and C-terminal domains of PrsA in Streptococcus suis type 2 are crucial for inducing cell death independent of TLR2 recognition [ Virulence, 2023, 14(1):2249779] PubMed: 37641974
Flanking N- and C-terminal domains of PrsA in Streptococcus suis type 2 are crucial for inducing cell death independent of TLR2 recognition [ Virulence, 2023, 14(1):2249779] PubMed: 37641974
Anti-ferroptotic PRKAA2 serves as a potential diagnostic and prognostic marker for hepatoblastoma [ J Gastrointest Oncol, 2023, 14(4):1788-1805] PubMed: 37720445

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。