Nocodazole

製品コードS2775 バッチS277502

印刷

化学情報

Chemical Structure Synonyms R17934, Oncodazole, NSC238159 Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

C14H11N3O3S
分子量 301.32 CAS No. 31430-18-9
Solubility (25°C)* 体外 DMSO 7.5 mg/mL (24.89 mM)
Water Insoluble
Ethanol Insoluble
体内 （毎回新しく調製した物を用意してください）
Clear solution
5%DMSO 40%PEG300 5%Tween80 50%ddH2O
0.38mg/ml Taking the 1 mL working solution as an example, add 50 μL 7.5 mg/ml clarified DMSO stock solution to 400 μL PEG300, mix evenly to clarify it; add 50 μL Tween80 to the above system, mix evenly to clarify it; then continue to add 500 μL ddH2O to make it clear. Volume up to 1 mL. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 Nocodazole is a rapidly-reversible inhibitor of microtubule polymerization, also inhibits Abl, Abl(E255K) and Abl(T315I) with IC50 of 0.21 μM, 0.53 μM and 0.64 μM in cell-free assays, respectively. Nocodazole induces apoptosis.
in vitro

Nocodazole is a high-affinity ligand for the cancer-related kinases including Abl phosphorylated, c-Kit, BRAF, and MEK with Kd of 0.091 μM, 1.6 μM, 1.8 μM and 1.6 μM, respectively. In addition, the Kd of Nocodazole for Abl(E255K) phosphorylated, Abl(T315I) phosphorylated, BRAF(V600E) and PI3Kγ is 0.12 μM, 0.17 μM, 1.1 μM and 1.5 μM, respectively. Nocodazole induces apoptosis in chronic lymphocytic leukemia cells. Nocodazole inhibits insulin-stimulated glucose transport. Nocodazole decreases apoptosis in some human colon carcinoma cells. Nocodazole impairs the morphology and directionality of migrating medial gan-glionic eminence cells. [1]

At high concentrations, Nocodazole rapidly depolymerizes microtubules in cells, while low concentrations of Nocodazole inhibit microtubule dynamic instability. [2]

Mitotic cells incubated with different concentrations of Paclitaxel are inhibited from progressing to G1 phase 6 hours after release from the Nocodazole block, with a median inhibitory concentration of 4 nM. Nocodazole-pretreated cells exposed to Paclitaxel in the absence of Nocodazole only form free-floating microtubules, whereas pretreated cells exposed to Paclitaxel in the presence of Nocodazole-assembled centrosome organize microtubules. [3]

Nocodazole disrupts microtubules by binding to β-tubulin. Nocodazole prevents the formation of one of the two interchain disulfide linkages. Nocodazole impairs the transport of vesicles. Nocodazole suppress METH-induced cell death and lysosomal dysfunction. METH-induced cell death is significantly decreased by Nocodazole pretreatment in comparison to METH alone. [4]

Nocodazole doubles HDR efficiency to up to 30% in iPSCs. It improves the CRISPR-mediated HDR efficiency and has an additive effect on enhancing precise genome editing[6].
in vivo

The tumor volume and tumor weight of the mice treated with Ketoconazole  plus  Nocodazole are significantly reduced as compared with those treated with Ketoconazole or Nocodazole alone. Combined treatment with Ketoconazole  plus  Nocodazole strongly enhances apoptosis of COLO 205 tumor xenografts treated with Ketoconazole  or  Nocodazole alone. [5]

プロトコル(参考用のみ)

細胞アッセイ 細胞株 H1975 cells
濃度 50 ng/ml
反応時間 24 h
実験の流れ

Cells were treated with indicated concentration of the drug for 24 hour.
動物実験 動物モデル Nude mice with COLO-205 tumor xenografts
投薬量 5 mg/kg
投与方法 Administered via i.p.

カスタマーフィードバック

, , J Biol Chem, 2016, 291:14761-14772.

Data from [Data independently produced by , , Cancer Res, 2018, doi:10.1158/0008-5472.CAN-18-0520]

Data from [Data independently produced by , , Cell Signal, 2016, 28(12):1826-1832]

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Nucleolar protein TAAP1/C22orf46 confers pro-survival signaling in non-small cell lung cancer [ Life Sci Alliance, 2024, 7(4)e202302257] PubMed: 38228372
Allosteric regulation of CAD modulates de novo pyrimidine synthesis during the cell cycle [ Nat Metab, 2023, 5(2):277-293] PubMed: 36747088
Real-Time Dissection of the Transportation and miRNA-Release Dynamics of Small Extracellular Vesicles [ Adv Sci (Weinh), 2023, 10(7):e2205566] PubMed: 36599707
Harnessing transcriptionally driven chromosomal instability adaptation to target therapy-refractory lethal prostate cancer [ Cell Rep Med, 2023, 4(2):100937] PubMed: 36787737
Loss of RanGAP1 drives chromosome instability and rapid tumorigenesis of osteosarcoma [ Dev Cell, 2023, 58(3):192-210.e11] PubMed: 36696903
Fatty acid oxidation facilitates DNA double-strand break repair by promoting PARP1 acetylation [ Cell Death Dis, 2023, 14(7):435] PubMed: 37454129
Targeting MCL-1 triggers DNA damage and an anti-proliferative response independent from apoptosis induction [ Cell Rep, 2023, 42(10):113176] PubMed: 37773750
Ferroptosis signaling promotes the release of misfolded proteins via exosomes to rescue ER stress in hepatocellular carcinoma [ Free Radic Biol Med, 2023, 202:110-120] PubMed: 36997100
PLK1 maintains DNA methylation and cell viability by regulating phosphorylation-dependent UHRF1 protein stability [ Cell Death Discov, 2023, 9(1):367] PubMed: 37788997
PLK1 maintains DNA methylation and cell viability by regulating phosphorylation-dependent UHRF1 protein stability [ Cell Death Discov, 2023, 9(1):367] PubMed: 37788997

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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