Nomilin

製品コードS5045 バッチS504501

印刷

化学情報

Chemical Structure Synonyms N/A Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

C28H34O9
分子量 514.56 CAS No. 1063-77-0
Solubility (25°C)* 体外 DMSO 100 mg/mL (194.34 mM)
Water Insoluble
Ethanol Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 Nomilin is a triterpenoid present in common edible citrus fruits with putative anticancer properties.
in vitro Administration of nomilin significantly retarded endothelial cell proliferation, migration, invasion and tube formation. It also possesses anti-proliferative activity against number of human cancer cell lines including leukemia (HL-60), ovary (SKOV-3), cervix (HeLa), stomach (NCI-SNU-1), liver (Hep G2), and breast (MCF-7)[2]. Nomilin significantly decreased TRAP-positive multinucleated cell numbers compared with the control, and exhibited no cytotoxicity. It decreases bone resorption activity and downregulates osteoclast-specific genes, NFATc1 and TRAP mRNA levels. Furthermore, nomilin suppressed MAPK signaling pathways. Thus, nomilin has inhibitory effects on osteoclastic differentiation in vitro[3].
in vivo Apart from antifeedant activity, Nomilin is a potent inducers of gluthathione S-transferase activity in mice and to inhibit carcinogenesis in the hamster buccal pouch assay. Nomilin can shorten anaesthetic-induced sleeping time in mice, probably through a stimulant activity on the central nervous system[1]. Nomilin significantly inhibited tumor directed capillary formation. Serum proinflammatory cytokines such as IL-1β, IL-6, TNF-α and GM-CSF and also serum NO levels were significantly reduced by the treatment of nomilin. Administration of nomilin significantly reduced the serum level of VEGF, a proangiogenic factor and increased the antiangiogenic factors IL-2 and TIMP-1[2].

プロトコル(参考用のみ)

細胞アッセイ 細胞株 Human umbilical vein endothelial cells (HUVECs)
濃度 5 μg-500 μg/ml
反応時間 48 h
実験の流れ

HUVECs were seeded (5000 cells/well) in 96-well flat bottomed titer plate and incubated for 24 h at 37 °C in 5% CO2 atmosphere. Different concentrations of nomilin (5 μg-500 μg/ml) were added and incubated further for 48 h. Before 4 h completion of incubation, 20 μl MTT (5 mg/ml) was added. Percentage of dead cells was determined using an ELISA plate reader set to record absorbance at 570 nm.
動物実験 動物モデル Four to six week old male C57BL/6 mice
投薬量 6 mg/kg
投与方法 i.p.

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。