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Synonyms | N/A | Storage (From the date of receipt) |
3 years -20°C powder | |
化学式 | C20H17N6NaO3 |
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分子量 | 412.38 | CAS No. | 6300-44-3 | |
Solubility (25°C)* | 体外 | DMSO | 82 mg/mL (198.84 mM) | |
Water | Insoluble | |||
Ethanol | Insoluble | |||
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. |
製品説明 | NSC45586 is an inhibitor of PHLPP (Pleckstrin homology domain and leucine-rich repeat protein phosphatase), which is selective for PHLPP1 and PHLPP2. |
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in vitro | PH domain and leucine rich repeat phosphatase (Phlpp) inhibitor NSC45586 increases Pth1r mRNA and protein expression in ATDC5 cells and IMCs within 24 hours. [2] |
細胞アッセイ | 細胞株 | ATDC5 cells, Primary immature chondrocytes (IMCs) |
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濃度 | 25 μM | |
反応時間 | 24 h | |
実験の流れ | ATDC5 cells were seeded at a density of 5 x 105 cells/well in a six-well plate and incubated overnight to allow for cell adhesion in DMEM supplemented with 10% FBS and 100 units/mL penicillin, 100 μg/mL streptomycin, and 0.25 μg/mL Amphotericin B (1% antibiotic/antimycotic). Phlppi including NSC45586 (25 μM) treatments were added. Primary chondrocytes were plated and allowed to adhere overnight as described above. Actinomycin D (5μM) was added for six hours and then replaced with medium containing Phlppi including NSC45586 or vehicle for 24 hours. Cells were collected for RNA or protein extraction. |
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長期の保管のために-20°Cの下で製品を保ってください。
人間や獣医の診断であるか治療的な使用のためにでない。
各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。