Oligomycin A

製品コードS1478 バッチS147805

化学情報

	Synonyms	MCH 32	Storage (From the date of receipt)	3 years -20°C powder 1 years -80°C in solvent
	化学式	C₄₅H₇₄O₁₁
	分子量	791.06	CAS No.	579-13-5
Solubility (25°C)*	体外	DMSO	15 mg/mL (18.96 mM)
		Water	Insoluble
		Ethanol	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

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生物活性

製品説明	Oligomycin A (MCH 32), a dominant analog of the isomers, is an inhibitor of mitochondrial F1FO ATP synthase which inhibits oxidative phosphorylation and all the ATP-dependent processes occurring on the coupling membrane of mitochondria. It inhibits ATP synthase by blocking its proton channel (Fo subunit), which is necessary for oxidative phosphorylation of ADP to ATP. It also induces apoptosis in a variety of cell types.
in vitro	Oligomycin A is an inhibitor of ATP synthase, inhibits oxidative phosphorylation and all the ATP-dependent processes occurring on the coupling membrane of mitochondria.Oligomycin A inhibits ATP synthase by blocking its proton channel (Fo subunit), which is necessary for oxidative phosphorylation of ADP to ATP. The inhibition of ATP synthesis by oligomycin A will significantly reduce electron flow through the electron transport chain; however, electron flow is not stopped completely due to a process known as proton leak or mitochondrial uncoupling. ^[1] In a group of cancer cells, Oligomycin at 100 ng/ml completely inhibits oxidative phosphorylation activity in 1 h and induces various levels of glycolysis gains by 6 h. ^[2] Oligomycin, inhibitor of the F0 part of H⁺-ATP-synthase, suppresses the TNF-induced apoptosis. ^[3] Oligomycin inhibits mitochondrial respiration in melanoma, sensitizes melanoma cells to therapy, blocks the emergence of the slow-cycling, long-term tumor-maintaining melanoma cells. ^[4]
in vivo	Oligomycin A (MCH 32) is an inhibitor of mitochondrial F0F1-ATPase.

製品説明

Oligomycin A (MCH 32), a dominant analog of the isomers, is an inhibitor of mitochondrial F1FO ATP synthase which inhibits oxidative phosphorylation and all the ATP-dependent processes occurring on the coupling membrane of mitochondria. It inhibits ATP synthase by blocking its proton channel (Fo subunit), which is necessary for oxidative phosphorylation of ADP to ATP. It also induces apoptosis in a variety of cell types.

in vitro

Oligomycin A is an inhibitor of ATP synthase, inhibits oxidative phosphorylation and all the ATP-dependent processes occurring on the coupling membrane of mitochondria.Oligomycin A inhibits ATP synthase by blocking its proton channel (Fo subunit), which is necessary for oxidative phosphorylation of ADP to ATP. The inhibition of ATP synthesis by oligomycin A will significantly reduce electron flow through the electron transport chain; however, electron flow is not stopped completely due to a process known as proton leak or mitochondrial uncoupling. ^[1]

In a group of cancer cells, Oligomycin at 100 ng/ml completely inhibits oxidative phosphorylation activity in 1 h and induces various levels of glycolysis gains by 6 h. ^[2]

Oligomycin, inhibitor of the F0 part of H⁺-ATP-synthase, suppresses the TNF-induced apoptosis. ^[3]

Oligomycin inhibits mitochondrial respiration in melanoma, sensitizes melanoma cells to therapy, blocks the emergence of the slow-cycling, long-term tumor-maintaining melanoma cells. ^[4]

in vivo

Oligomycin A (MCH 32) is an inhibitor of mitochondrial F0F1-ATPase.

プロトコル(参考用のみ)

細胞アッセイ	細胞株	H1229
	濃度	100 ng/ml
	反応時間	1 h
	実験の流れ	ATP and Oligomycin Dose-Response Growth Measurement. Cellular ATP changes are measured by CellTiter-Glo reagent. To measure oligomycin dose-response curves, the cells are plated in 96-well plates at about 400–500 cells/well in 100 μl of culture, dosed the next day, and grown for 4 additional days followed by assaying with Cell-Titer-Glo reagent. The dose-response curves are plotted with nonlinear regression analysis of GraphPad Prism.
動物実験	動物モデル	Male Sprague-Dawley rats
	投薬量	0.5 mg/kg
	投与方法	i.v.

参考

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カスタマーフィードバック

: Data from [Data independently produced by , , Sci Rep, 2016, 6:24641.]

: Data from [Data independently produced by , , Int J Oncol, 2018, 53(6):2590-2604]

: Data from [Data independently produced by , , PLoS One, 2017, 12(4):e0176355]

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長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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