PACAP 1-38

製品コードS8415 バッチS841502

印刷

化学情報

Chemical Structure Synonyms Pituitary Adenylate Cyclase Activating Polypeptide 38 Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

C203H331N63O53S
分子量 4534.26 CAS No. 137061-48-4
Solubility (25°C)* 体外 Water 100 mg/mL (22.05 mM)
 
 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 PACAP 1-38 (Pituitary Adenylate Cyclase Activating Polypeptide 38) is a highly potent PACAP receptor agonist (Kd = 100 pM). It stimulates adenylate cyclase and phagocytosis.
in vitro PACAP 1-38 has potent, efficacious, and sustained stimulatory effects on sympathetic neuronal NPY and catecholamine production[1]. It is a pleiotropic neuropeptide, exhibiting a variety of biologic actions, including activities as a neurotransmitter, neuromodulator, neurotrophic factor, as well as an immunomodulator, in immune cells through its effect on MAPK signaling and modulation of activation of NFκB. PACAP 1-38 dramatically prevents injury of cultured renal proximal tubule cells caused by myeloma light chains through suppression of proinflammatory cytokines production, by inhibiting p38 MAPK and translocation of NFκB via both PAC1 and VPAC1 receptors. PACAP38 inhibits myeloma cell growth directly and may also indirectly by suppressing production of the growth factor, IL-6, from bone marrow stromal cells, that is stimulated by adhesion of myeloma cells. PACAP38 suppressed release of both IL-6 and TNFα dose dependently[2].
in vivo PACAP38 is capable of inhibiting light chain-induced cytokine expression with a great potency and prevented the resulting cell damage in vivo. However, PACAP is also considered as an autoregulatory factor for certain tumors, stimulating their growth in an autocrine fashion[2].

プロトコル(参考用のみ)

細胞アッセイ 細胞株 human renal proximal tubule cell line
濃度 0.0001-100 nM
反応時間 3 days
実験の流れ

Human renal proximal tubule cells plated onto 6-well tissue culture plates are grown at 37°C in DRM-23E medium supplemented with 0.5% (vol/vol) FBS in an incubator for 24 hours. After prewashing with serum-free medium, the cells are incubated with κ-LC (1.5 mg/ml, ∼ 50 μM) for 3 days in the presence and absence of various concentrations of PACAP38 or dexamethasone as well as kinase and transcription factor inhibitors. Cell viability is determined by trypan blue exclusion assays; in all experiments, at least 85% of cells remain viable. After exposure to test substances, culture supernatants are harvested and stored at –70°C for cytokine assays. After the medium is removed, the cells are washed with ice-cold PBS, and proteins are extracted by lysing cells with Sigma Mammalian Cell Lysis Reagents. Lysates are scraped and passed through a 21-gauge needle to shear DNA, and centrifuged at 12 000g for 10 minutes at 4°C. Finally, supernatants are harvested and used for kinase studies.
動物実験 動物モデル Male Sprague-Dawley rats
投薬量 2 nmol/10 mL
投与方法 i.v.

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Intravitreal Injection of PACAP Attenuates Acute Ocular Hypertension-Induced Retinal Injury Via Anti-Apoptosis and Anti-Inflammation in Mice [ Invest Ophthalmol Vis Sci, 2022, 63(3):18] PubMed: 35293951
PAC1 Receptor Mediates Electroacupuncture-Induced Neuro and Immune Protection During Cisplatin Chemotherapy [ Front Immunol, 2021, 12:714244] PubMed: 34552585
PACAP causes PAC1/VPAC2 receptor mediated hypertension and sympathoexcitation in normal and hypertensive rats [ Am J Physiol Heart Circ Physiol, 2012, 303(7):H910-7] PubMed: 22886412
PACAP causes PAC1/VPAC2receptor mediated hypertension and sympathoexcitation in normal and hypertensive rats [ American Journal of Physiology-Heart and Circulatory Physiology, 2012, Vol. 303, No. 7] PubMed: None

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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