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Synonyms | (-)-Patchouli Alcohol, Patchoulol, Patchouli camphor | Storage (From the date of receipt) |
3 years -20°C powder 1 years -80°C in solvent |
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化学式 | C15H26O |
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分子量 | 222.37 | CAS No. | 5986-55-0 | |
Solubility (25°C)* | 体外 | DMSO | 44 mg/mL (197.86 mM) | |
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. |
製品説明 | Patchouli alcohol (Patchoulol, Patchouli camphor), an ingredient in pogostemonis herba, has various pharmacological activities and acts as an inhibitor of asexual fungal propagation, inflammation, the influenza virus, and tumorigenesis. |
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in vitro | Patchouli alcohol inhibits the overexpression of iNOS and IL-6 in protein and mRNA levels in LPS-stimulated RAW264.7 and TNF-a stimulated HT-29 cells. Patchouli alcohol inhibits IkB-α degradation and p65 nuclear translocation, and subsequently suppresses transcriptional activity of NF-kB in LPS-stimulated RAW264.7 and TNF-α-stimulated HT-29 cells. In addition, Patchouli alcohol inhibits LPS- or TNF-α-stimulated ERK1/2 activation by decreasing phosphorylation of ERK1/2[1]. |
細胞アッセイ | 細胞株 | RAW264.7 and HT-29 cells |
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濃度 | 50 and 75 μM | |
反応時間 | 21 h | |
実験の流れ | RAW264.7 and HT-29 cells (2×105 cells/well) are seeded in 12-well plates and incubated overnight. Then, plasmid mixtures containing 0.5 μg of pNF-kB-Luc plasmid and 0.1 μg of pRL-null vector are transfected for 24 h (for RAW264.7) and 48 h (for HT-29). The empty pGL3-basic vector is used as a negative control. After transfection, cells are pre-treated with Patchouli alcohol, tolfenamic acid (TA), or sulindac sulfide (SS) for 6 h and then co-treated with LPS (for RAW264.7) or TNF-α (for HT-29) for an additional 15 h. The cells are harvested in 1×luciferase lysis buffer, and luciferase activity is measured and normalized to the pRL-null luciferase activity using a dual-luciferase assay kit. |
長期の保管のために-20°Cの下で製品を保ってください。
人間や獣医の診断であるか治療的な使用のためにでない。
各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。