PFM01

製品コードS3549 バッチS354901

化学情報

	Synonyms	N/A	Storage (From the date of receipt)	3 years -20°C powder
	化学式	C₁₄H₁₅NO₂S₂
	分子量	293.40	CAS No.	1558598-41-6
Solubility (25°C)*	体外	DMSO	59 mg/mL (201.09 mM)
		Ethanol	59 mg/mL (201.09 mM)
		Water	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	PFM01, a cell-permeable N-alkylated Mirin derivative, is a selective inhibitor of MRE11 endonuclease activity.
in vitro	PFM01 rescues the repair defect in 48BR (WT) and HSC62 (BRCA2-defective) primary cells, diminishes the RAD51 foci formation in 1BR3 (WT) and HSC62 (BRCA2-defective) cells, enhances non-homologous end-joining (NHEJ) in H1299 dA3 cells and reduces homologous recombination (HR) in U2OS DR-GFP cells, also substantially relieves the double-strand break (DSB) repair defect confers by mirin or PFM39 in irradiated G2 cells.^[1]

製品説明

PFM01, a cell-permeable N-alkylated Mirin derivative, is a selective inhibitor of MRE11 endonuclease activity.

in vitro

PFM01 rescues the repair defect in 48BR (WT) and HSC62 (BRCA2-defective) primary cells, diminishes the RAD51 foci formation in 1BR3 (WT) and HSC62 (BRCA2-defective) cells, enhances non-homologous end-joining (NHEJ) in H1299 dA3 cells and reduces homologous recombination (HR) in U2OS DR-GFP cells, also substantially relieves the double-strand break (DSB) repair defect confers by mirin or PFM39 in irradiated G2 cells.^[1]

プロトコル(参考用のみ)

細胞アッセイ	細胞株	48BR (human primary fibroblasts), ATLD2 (primary fibroblasts), 1BR3 hTERT, HSC62 hTERT (BRCA2-defective) and 2BN hTERT (XLF-defective)
	濃度	30 min
	反応時間	100 μM
	実験の流れ	48BR (human primary fibroblasts), ATLD2 (primary fibroblasts), 1BR3 hTERT, HSC62 hTERT (BRCA2-defective) and 2BN hTERT (XLF-defective), were cultured in Dulbecco’s modification of Eagles medium with 15% fetal calf serum (FCS). A549 cells were cultured in minimal essential medium (MEM) with 10% FCS. Cells were irradiated in medium using a ¹³⁷Cs source. MRE11 inhibitor concentrations were 500 μM Mirin, 100 μM PFM39, 100 μM PFM01 and 100 μM PFM03, unless otherwise stated. 10 μM ATM inhibitor (KU55933) or 10 μM DNA-PKcs inhibitor (NU7441) were added 30 min prior to irradiation unless indicated.

参考

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

ATR protects ongoing and newly assembled DNA replication forks through distinct mechanisms [ Cell Rep, 2023, 42(7):112792]	PubMed: 37454295

ATR protects ongoing and newly assembled DNA replication forks through distinct mechanisms [ Cell Rep, 2023, 42(7):112792]

PubMed: 37454295

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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