PMA (Phorbol 12-myristate 13-acetate)

製品コードS7791 バッチS779104

化学情報

	Synonyms	12-O-Tetradecanoylphorbol-13-acetate,TPA, Phorbol myristate acetate	Storage (From the date of receipt)	3 years -20°C(in the dark) powder
	化学式	C₃₆H₅₆O₈
	分子量	616.83	CAS No.	16561-29-8
Solubility (25°C)*	体外	DMSO	100 mg/mL (162.11 mM)
		Ethanol	100 mg/mL (162.11 mM)
		Water	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	PMA (Phorbol 12-myristate 13-acetate), a potent activator of PKC, is active at nanomolar concentrations. Phorbol 12-myristate 13-acetate (PMA) induces sphingosine-1-phosphate (S1P).
in vitro	In endothelial cells, PMA increases levels of Thy-1 mRNA and protein, induces Thy-1 up-regulation and inhibits capillary-like tube formation and endothelial cell migration.^[1]
in vivo	In the zebrafish model, PMA initially enhances endothelial cell migration, subsequently activates the PKC-δ/Syk/NF-κB-mediated pathway to up-regulate Thy-1, which in turn inhibits endothelial cell migration.^[1]

プロトコル(参考用のみ)

細胞アッセイ	細胞株	Human umbilical vein endothelial cells (HUVECs), human dermal microvascular endothelial cells (HDMECs), human pulmonary microvascular endothelial cell line (HPMECs)
	濃度	20 ng/mL
	反応時間	14, 16 and 18 h for levels of Ty-1 mRNA, 20, 24 and 28 h for levels of Ty-1 protein
	実験の流れ	After transfection with Ty-1 promoter plasmid (0.5 μg) and Renilla plasmid (50ng), the cells are treated with PMA (20ng/mL), and washed with ice-cold phosphate-buffered saline (PBS), and then lysed in passive lysis buffer. Luciferase activity is measured using the Dual-Luciferase Reporter Assay kit.
動物実験	動物モデル	Transgenic Tg(fi1:EGFP)y1 embryos of zebrafsh (Danio rerio)
	投薬量	20 ng/mL
	投与方法	--

参考

[1] Wen HC, et al. Sci Rep. 2018 Nov 2;8(1):16247.

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長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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