受注:045-509-1970 |
技術サポート:[email protected] 平日9:00〜18:00 1営業日以内にご連絡を差し上げます |
Synonyms | 12-O-Tetradecanoylphorbol-13-acetate,TPA, Phorbol myristate acetate | Storage (From the date of receipt) |
3 years -20°C(in the dark) powder | |
化学式 | C36H56O8 |
|||
分子量 | 616.83 | CAS No. | 16561-29-8 | |
Solubility (25°C)* | 体外 | DMSO | 100 mg/mL (162.11 mM) | |
Ethanol | 100 mg/mL (162.11 mM) | |||
Water | Insoluble | |||
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. |
製品説明 | PMA (Phorbol 12-myristate 13-acetate), a potent activator of PKC, is active at nanomolar concentrations. Phorbol 12-myristate 13-acetate (PMA) induces sphingosine-1-phosphate (S1P). |
---|---|
in vitro | In endothelial cells, PMA increases levels of Thy-1 mRNA and protein, induces Thy-1 up-regulation and inhibits capillary-like tube formation and endothelial cell migration.[1] |
in vivo | In the zebrafish model, PMA initially enhances endothelial cell migration, subsequently activates the PKC-δ/Syk/NF-κB-mediated pathway to up-regulate Thy-1, which in turn inhibits endothelial cell migration.[1] |
細胞アッセイ | 細胞株 | Human umbilical vein endothelial cells (HUVECs), human dermal microvascular endothelial cells (HDMECs), human pulmonary microvascular endothelial cell line (HPMECs) |
---|---|---|
濃度 | 20 ng/mL | |
反応時間 | 14, 16 and 18 h for levels of Ty-1 mRNA, 20, 24 and 28 h for levels of Ty-1 protein | |
実験の流れ | After transfection with Ty-1 promoter plasmid (0.5 μg) and Renilla plasmid (50ng), the cells are treated with PMA (20ng/mL), and washed with ice-cold phosphate-buffered saline (PBS), and then lysed in passive lysis buffer. Luciferase activity is measured using the Dual-Luciferase Reporter Assay kit. |
|
動物実験 | 動物モデル | Transgenic Tg(fi1:EGFP)y1 embryos of zebrafsh (Danio rerio) |
投薬量 | 20 ng/mL | |
投与方法 | -- |
ALIX and TSG101 are essential for cellular entry and replication of two porcine alphacoronaviruses [ PLoS Pathog, 2024, 20(3):e1012103] | PubMed: 38489378 |
Glutamine metabolic microenvironment drives M2 macrophage polarization to mediate trastuzumab resistance in HER2-positive gastric cancer [ Cancer Commun (Lond), 2023, 43(8):909-937] | PubMed: 37434399 |
Targeting TCF19 sensitizes MSI endometrial cancer to anti-PD-1 therapy by alleviating CD8+ T cell exhaustion via TRIM14-IFN-β axis [ Cell Rep, 2023, 42(8):112944] | PubMed: 37566545 |
Transferred mitochondria accumulate reactive oxygen species, promoting proliferation [ Elife, 2023, 12e85494] | PubMed: 36876914 |
Transferred mitochondria accumulate reactive oxygen species, promoting proliferation [ Elife, 2023, 10.7554/eLife.85494] | PubMed: 36876914 |
Regulation of PD-L1 expression in non-small cell lung cancer by interleukin-1β [ Front Immunol, 2023, 14:1192861] | PubMed: 37441079 |
Stimulation of antitumor immunity by FoxP3-targeting PROTAC [ Biomed Pharmacother, 2023, 163:114871] | PubMed: 37182514 |
Tumor-derived interleukin-1 receptor antagonist exhibits immunosuppressive functions and promotes pancreatic cancer [ Cell Biosci, 2023, 13(1):147] | PubMed: 37563620 |
A potential anti-HIV-1 compound, Q308, inhibits HSV-2 infection and replication in vitro and in vivo [ Biomed Pharmacother, 2023, 162:114595] | PubMed: 36989723 |
Regulation of PD-L1 expression in non-small cell lung cancer by interleukin-1β [ Front Immunol, 2023, 14:1192861] | PubMed: 37441079 |
長期の保管のために-20°Cの下で製品を保ってください。
人間や獣医の診断であるか治療的な使用のためにでない。
各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。