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Synonyms | N/A | Storage (From the date of receipt) |
3 years -20°C powder | |
化学式 | C20H16O5 |
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分子量 | 336.34 | CAS No. | 18642-23-4 | |
Solubility (25°C)* | 体外 | DMSO | 67 mg/mL (199.2 mM) | |
Ethanol | 1 mg/mL (2.97 mM) | |||
Water | Insoluble | |||
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. |
製品説明 | Psoralidin, a naturally occurring coumestan isolated from the fractions of organic solvents such as ethylacetate, hexane, or n-butanol of the seed extract of Psoralea corylifolia L., has a variety of biological activities such as anticancer, antioxidant, antibacterial, antidepressant, anti-inflammatory activities, and regulation of insulin signaling. It is an agonist for both estrogen receptor (ER)α and ERβ with binding affinities (IC50s) of 1.03 and 24.6 μM, respectively. |
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in vitro | Psoralidin has been characterized as a full ER agonist, which activates the classical ER-signaling pathway in both ER-positive human breast and endometrial cell lines as well as non-human cultured cells transiently expressing either ERα or ERβ. Psoralidin was also able to induce the endogenous estrogen-responsive gene, pS2, in human breast cancer cells MCF-7. The IC50 values for psoralidin to replace the binding of E2 to both receptors were 1.03 and 24.6 μM, respectively. Psoralidin is able to bind to both receptors within the micromolar range and has preferential affinity for ERα over ERβ[1]. |
in vivo | Acute and subchronic administrations of psoralidin produced a significant reduction in immobility time in the mouse forced swimming test. Psoralidin dose-dependently increased swimming and failed to affect climbing[2]. |
細胞アッセイ | 細胞株 | MCF-7 cells |
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濃度 | 10 μM | |
反応時間 | 24 h | |
実験の流れ | MCF-7 cells were cultured in estrogen-free RPMI 1640 media for 4 days before transfection, and plated (4 × 105 cells/well) in triplicate onto a 12-well plate. Cells were transiently transfected with pERE-luciferase plasmid (0.5 μg/well) and with an internal control plasmid pRL-Tk (0.1 μg/well) using Lipofectamine 2000 Reagent. ERE-luciferase plasmid contains three copies of the Xenopus laevis vitellogenin A2 ERE upstream of fire fly luciferase. The pRL-Tk plasmid contains a cDNA encoding Renilla luciferase. At 24 h post-transfection, cells were treated DMSO, various concentrations of E2, psoralidin, ICI, or appropriate combination of two compounds and incubated for another 24 h. The cells were harvested with Passive Lysis buffer. Luciferase activity present in the cell lysates was measured. |
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動物実験 | 動物モデル | Male ICR strain of mice |
投薬量 | 20, 40 and 60 mg/kg | |
投与方法 | oral |
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長期の保管のために-20°Cの下で製品を保ってください。
人間や獣医の診断であるか治療的な使用のためにでない。
各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。