Pyridostatin Trifluoroacetate Salt

製品コードS7444 バッチS744403

化学情報

	Synonyms	RR82 Trifluoroacetate Salt	Storage (From the date of receipt)	3 years -20°C powder 1 years -80°C in solvent
	化学式	C₃₁H₃₂N₈O₅·C₂HF₃O₂
	分子量	710.7	CAS No.	1472611-44-1
Solubility (25°C)*	体外	DMSO	100 mg/mL (140.7 mM)
		Water	30 mg/mL (42.21 mM)
		Ethanol	9 mg/mL (12.66 mM)
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	Pyridostatin Trifluoroacetate Salt is a G-quadruplexe stabilizer with K_d of 490 nM in a cell-free assay, which targets a series of proto-oncogenes.
in vitro	Pyridostatin decreases the proliferation of MRC-5–SV40 cells and various cancer cell lines, and induces cell-cycle arrest by DNA-damage checkpoint activation. Pyridostatin also reduces SRC-dependent cell motility in MDA-MB-231 cells by interacting with G-quadruplex motifs in SRC. ^[2] Pyridostatin decreases EBNA1 synthesis via inhibition of G-quadruplexes. ^[3]
in vivo

製品説明

Pyridostatin Trifluoroacetate Salt is a G-quadruplexe stabilizer with K_d of 490 nM in a cell-free assay, which targets a series of proto-oncogenes.

in vitro

Pyridostatin decreases the proliferation of MRC-5–SV40 cells and various cancer cell lines, and induces cell-cycle arrest by DNA-damage checkpoint activation. Pyridostatin also reduces SRC-dependent cell motility in MDA-MB-231 cells by interacting with G-quadruplex motifs in SRC. ^[2]

Pyridostatin decreases EBNA1 synthesis via inhibition of G-quadruplexes. ^[3]

in vivo

プロトコル(参考用のみ)

キナーゼアッセイ
キナーゼアッセイ
細胞アッセイ	細胞株	MRC-5–SV40 cells and various cancer cell lines
	濃度	10 μM
	反応時間	48 h
	実験の流れ	Cells are plated at equal confluence and are left either untreated or were treated with 2 μM pyridostatin continually during the indicated time before harvesting the cells. Cells from individual plates are trypsinized and counted in a Coulter counter. Graphs represent the total cell numbers at each time interval, and the error bars represent the s.e.m. Data represent three independent experiments.
動物実験	動物モデル	CB17‐SCID mice
	投薬量	7.5 mg/kg
	投与方法	i.v.

参考

[4] Groelly FJ, et al. EMBO Mol Med. 2022 Mar 7;14(3):e14501.

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Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

RAD54L2-mediated DNA damage avoidance pathway specifically preserves genome integrity in response to topoisomerase 2 poisons [ Sci Adv, 2023, 9(49):eadi6681]	PubMed: 38055811
G-quadruplex binders as cytostatic modulators of innate immune genes in cancer cells [ Nucleic Acids Res, 2021, gkab500]	PubMed: 34139015
Differential responses of neurons, astrocytes, and microglia to G-quadruplex stabilization [ Aging (Albany NY), 2021, 13(12):15917-15941]	PubMed: 34139671
Characterization of an active LINE-1 in the naked mole-rat genome [ Sci Rep, 2021, 11(1):5725]	PubMed: 33707548
hnRNP H/F Drive RNA G-quadruplex-mediated Translation Linked to Genomic Instability and Therapy Resistance in Glioblastoma [ Nat Commun, 2020, 27;11(1):2661]	PubMed: 32461552
Replication Stress Induces ATR/CHK1-Dependent Nonrandom Segregation of Damaged Chromosomes. [ Mol Cell, 2020, 22 pii: S1097-2765(20)30231-8]	PubMed: 32353258
Regulation of Autophagy by DNA G-quadruplexes [ Autophagy, 2020, 18]	PubMed: 32420812
HNRNPH1-dependent splicing of a fusion oncogene reveals a targetable RNA G-quadruplex interaction [ RNA, 2019, 25(12):1731-1750]	PubMed: 31511320
HNRNPH1-dependent splicing of a fusion oncogene reveals a targetable RNA G-quadruplex interaction [ RNA, 2019, 25(12):1731-1750]	PubMed: 31511320

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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