Q-VD-Oph

製品コードS7311 バッチS731104

印刷

化学情報

Chemical Structure Synonyms Quinoline-Val-Asp-Difluorophenoxymethylketone Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式
C26H25F2N3O6
分子量 513.49 CAS No. 1135695-98-5
Solubility (25°C)* 体外 DMSO 100 mg/mL (194.74 mM)
Ethanol 100 mg/mL (194.74 mM)
Water Insoluble
体内 （毎回新しく調製した物を用意してください）
Clear solution
5%DMSO 40%PEG300 5%Tween80 50%ddH2O
5.0mg/ml Taking the 1 mL working solution as an example, add 50 μL of 100 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to make it clear; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 Q-VD-Oph (Quinoline-Val-Asp-Difluorophenoxymethylketone) is a potent pan-caspase inhibitor with IC50 ranged from 25 to 400 nM for caspases 1,3,8, and 9. Q-VD-OPh can inhibits HIV infection.
in vitro Q-VD-OPh （5-100 μM） potently inhibits Actinomycin D-induced DNA laddering and subsequent apoptosis with minimal toxicity in WEHI 231 cells. Q-VD-OPh prevents caspase mediated cleavage of PARP and activation of the major initiator and effector caspases. [2] Q-VD-OPh protects cardiac myocytes from virus-induced apoptosis in vitro. [4]
in vivo Q-VD-OPh inhibits caspase-1 activity, IL-18 protein expression, and neutrophil infiltration during ischemic ARF in mice. [3] In vivo, caspase inhibition by Q-VD-OPh provides protection from virus-induced myocardial injury, with a significant reduction in caspase-3 activity. [4] In TgCRND8 mice, Q-VD-OPh inhibits caspase-7 activation and limits the pathological changes associated with tau, including caspase cleavage. [5]

プロトコル(参考用のみ)

細胞アッセイ 細胞株 WEHI-231 cells
濃度 ~1000 μM
反応時間 4 hours
実験の流れ Caspase inhibitors are added at the indicated concentrations 30 minutes prior to the addition of apoptotic stimuli. Viability and cell number are determined by trypan blue exclusion from three random fields of greater than 200 cells/field. All experiments are performed a minimum of three times.
動物実験 動物モデル C57BL/6 mice
投薬量 ~120 mg/kg
投与方法 Intraperitoneal administration

カスタマーフィードバック

Data from [Data independently produced by , , Mol Nutr Food Res, 2017, 61(12)]

Data from [Data independently produced by , , Neurochem Res, 2018, 43(6):1200-1209]

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Mitophagy curtails cytosolic mtDNA-dependent activation of cGAS/STING inflammation during aging [ Nat Commun, 2024, 15(1):830] PubMed: 38280852
Cancer Drug Resistance: Targeting Proliferation or Programmed Cell Death [ Cells, 2024, 13(5)388] PubMed: 38474352
The clarithromycin-binding proteins NIPSNAP1 and 2 regulate cytokine production through mitochondrial quality control [ Sci Rep, 2024, 14(1):2354] PubMed: 38287119
Treatment with siRNAs is commonly associated with GPX4 up-regulation and target knockdown-independent sensitization to ferroptosis [ Sci Adv, 2024, 10(11):eadk7329] PubMed: 38489367
Novel meriolin derivatives potently inhibit cell cycle progression and transcription in leukemia and lymphoma cells via inhibition of cyclin-dependent kinases (CDKs) [ Research Square, 2024, 10.21203/rs.3.rs-3857577/v1] PubMed: none
KLRG1 expression on natural killer cells is associated with HIV persistence, and its targeting promotes the reduction of the viral reservoir [ Cell Rep Med, 2023, 4(10):101202] PubMed: 37741278
KLRG1 expression on natural killer cells is associated with HIV persistence, and its targeting promotes the reduction of the viral reservoir [ Cell Rep Med, 2023, S2666-3791(23)00369-5] PubMed: 37741278
Dihydroartemisinin-induced ferroptosis in acute myeloid leukemia: links to iron metabolism and metallothionein [ Cell Death Discov, 2023, 9(1):97] PubMed: 36928207
Ebola virus shed glycoprotein is toxic to human T, B, and natural killer lymphocytes [ iScience, 2023, 26(8):107323] PubMed: 37529105
Ebola virus shed glycoprotein is toxic to human T, B, and natural killer lymphocytes [ iScience, 2023, 10.1016/j.isci.2023.107323] PubMed: 37529105

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。