SC75741

製品コードS7273 バッチS727301

印刷

化学情報

Chemical Structure Synonyms N/A Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

C29H23N7O2S2
分子量 565.67 CAS No. 913822-46-5
Solubility (25°C)* 体外 DMSO 41 mg/mL warmed with 50ºC water bath (72.48 mM)
Water Insoluble
Ethanol Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 SC75741 is a potent NF-κB inhibitor with EC50 of 200 nM. SC75741 efficiently blocks influenza virus propagation.
in vitro SC75741 shows immunosuppressive activity by inhibiting human PBMC proliferation with IC50 of 2.2 μM. [1] SC75741 inhibits replication of influenza A and B viruses by inhibiting NF-κB-mediated signalling on a transcriptional level. Moreover, SC75741 shows a high barrier for development of resistant virus variants. [2]
in vivo SC75741 (15 mg/kg i.p.) reduces virus replication and cytokine expression in mice lungs after H5N1 influenza virus infection. [2]
特徴 NF-κB-selective inhibitor.

プロトコル(参考用のみ)

キナーゼアッセイ NF-κB reporter gene assay
The NF-κB reporter gene assay is prepared with A549-NF-κB-SEAP cell line according to manufacturer’s instructions. In short, A549 cells stably transfected with pNF-κB-SEAP reporter gene plasmid are plated and allowed to attach overnight. The cells are subsequently incubated for 5 h with described compounds at 100, 30, 10, 3, 1, 0.3, 0.1, and 0 μM and then stimulated with 10 ng/ml TNF-α for 22 h. The supernatant of the cell is analyzed for SEAP activity using a chemiluminescent SEAP reporter gene assay.
細胞アッセイ 細胞株 Human PBMCs
濃度 ~50 μM
反応時間 48 hours
実験の流れ Cell viability assay is prepared using a CellTiter-BluTM Cell Viability Assay. For each concentration of the compound four replicates are measured.
動物実験 動物モデル C57BL/6 mice infected with H5N1 influenza virus
投薬量 ~15 mg/kg
投与方法 i.p.

カスタマーフィードバック

Data from [Data independently produced by , , Oncotarget, 2016, 7(32):52281-52293]

Data from [Data independently produced by , , J Nutr Biochem, 2017, 41:56-64]

Data from [Data independently produced by , , J Nutr Biochem, 2016, 41:56-64. ]

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Inhibiting the glycerophosphodiesterase EDI3 in ER-HER2+ breast cancer cells resistant to HER2-targeted therapy reduces viability and tumour growth [ J Exp Clin Cancer Res, 2023, 42(1):25] PubMed: 36670508
A Disintegrin and Metalloproteinase with Thrombospondin Motifs 4 Regulates Pulmonary Vascular Hyperpermeability through Destruction of Glycocalyx in Acute Respiratory Distress Syndrome [ Int J Mol Sci, 2023, 10.3390/ijms242216230] PubMed: 38003418
Development of a nanoparticle-based immunotherapy targeting PD-L1 and PLK1 for lung cancer treatment [ Nat Commun, 2022, 13(1):4261] PubMed: 35871223
Development of a nanoparticle-based immunotherapy targeting PD-L1 and PLK1 for lung cancer treatment [ Nat Commun, 2022, 13(1):4261] PubMed: 35871223
Oocytes could rearrange immunoglobulin production to survive over adverse environmental stimuli [ Front Immunol, 2022, 13:990077] PubMed: 36405746
Oocytes could rearrange immunoglobulin production to survive over adverse environmental stimuli [ Front Immunol, 2022, 13:990077] PubMed: 36405746
Homogeneous Polyporus Polysaccharide Inhibit Bladder Cancer by Resetting Tumor-Associated Macrophages Toward M1 Through NF-κB/NLRP3 Signaling [ Front Immunol, 2022, 13:839460] PubMed: 35603205
IFNγ directly counteracts imatinib-induced apoptosis of primary human CD34+ CML stem/progenitor cells potentially through the upregulation of multiple key survival factors [ Oncoimmunology, 2022, 11(1):2109861] PubMed: 35979386
Tumor-Derived C-C Motif Ligand 2 Induces the Recruitment and Polarization of Tumor-Associated Macrophages and Increases the Metastatic Potential of Bladder Cancer Cells in the Postirradiated Microenvironment [ Int J Radiat Oncol Biol Phys, 2022, S0360-3016(22)00598-3] PubMed: 35691449
Withaphysalins from Medicinal and Edible Physalis minima and Their Anti-inflammatory Activities [ J Agric Food Chem, 2022, 70(18):5595-5609] PubMed: 35476903

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。