SF1670

製品コードS7310 バッチS731001

印刷

化学情報

Chemical Structure Synonyms N/A Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

C19H17NO3
分子量 307.34 CAS No. 345630-40-2
Solubility (25°C)* 体外 DMSO (warmed with 50ºC water bath) 29 mg/mL (94.35 mM)
Ethanol (warmed with 50ºC water bath) 1 mg/mL (3.25 mM)
Water Insoluble
体内 （毎回新しく調製した物を用意してください）
Clear solution
5%DMSO 40%PEG300 5%Tween80 50%ddH2O
0.13mg/ml Taking the 1 mL working solution as an example, add 50 μL 2.5 mg/ml clarified DMSO stock solution to 400 μL PEG300, mix evenly to clarify it; add 50 μL Tween80 to the above system, mix evenly to clarify it; then continue to add 500 μL ddH2O to make it clear. Volume up to 1 mL. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 SF1670 is a highly potent and specific PTEN inhibitor with IC50 of 2 μM.
in vitro SF1670 shows potent cytotoxicity in HBEC, PC-3, H1299 cells with IC50 of 5 μM, 10 μM, 44 μM, respectively. In a mouse aortic ring matrigel angiogenesis model, SF1670 stimulates the angiogenic processes. [1] SF1670 augments chemoattractant-elicited PtdIns(3,4,5)P3 signaling in neutrophils and enhances neutrophil functions. [2]
in vivo Pretreatment of SF1670 (500 nM i.v.) augments bacteria-killing capability in neutropenic mice in both peritonitis and bacterial pneumonia, and decreases the mortality of neutropenia-related pneumonia. [2]

プロトコル(参考用のみ)

キナーゼアッセイ PTEN Inhibition Assay
To determine the dose response of potential PTEN inhibitors, doses of test compounds ranging from 1 nM to 250 uM (final reaction mix concentrations) are evaluated in the general PTEN inhibition assay. To obtain performed IC50 data, two separate rounds of the dose response assay are performed. In the first round, PTEN activity is tested in the presence of inhibitor at 10 fold serial dilutions ranging from 1 nM to 250 uM. Once the concentration range is determined, at which PTEN activity changes dramatically, two additional concentration data points within this range are added and the PTEN inhibition assay is then rerun for the second round. The PTEN inhibition IC50 is presented as the inhibitor concentration at which 50% of the PTEN activity. When the assay was run on multiple occasions and gave slightly different IC50 then those are reported as a range of IC50 found.
細胞アッセイ 細胞株 Human brain endothelial cells (HBEC), human prostate cancer cells (PC-3) and human non-small cell lung cancer cells (H1299)
濃度 ~1 mM
反応時間 2 hours
実験の流れ Cells are plated into 96-well plates in RPMI 1640 medium supplemented with 10% FBS and incubated overnight at 37°C. in an incubator containing an atmosphere of 5% CO2. The following day, the medium is replaced and cells are starved by placing in 100 uL of serum-free medium for 3 hours. Serially diluted test compounds are added to the wells and incubated with the cells for 2 hours at 37°C. Compounds are tested in a range from 1 mM to 0.1 nM depending on solubility. MTT is added to the wells at a final concentration of 5 μg/ml and incubated with the cells for 3 more hours. At the end of the incubation, the medium is aspirated and the MTT stain in the cells is dissolved by the addition of 100 μL DMSO. Optical density of each well is then measured at 570 nm using a SpectroMax Plus spectrophotometric plate reader. The IC50 determination from the data was made using Prism software.
動物実験 動物モデル Neutropenic mice
投薬量 500 nM
投与方法 Tail-vein injection

カスタマーフィードバック

Data from [Data independently produced by , , Int J Oncol, 2017, 51(3):899-906]

Data from [Data independently produced by , , Biochem Biophys Res Commun, 2017, 483(1):712-717]

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

TAN (tannic acid) inhibits BPA-induced pyroptosis of L8824 (grass carp hepatocytes) by regulating PTEN/PI3K/AKT pathway [ Fish Shellfish Immunol, 2024, 146:109384] PubMed: 38246267
Nicotine Decreases Nerve Regeneration and Pain Behaviors via PTEN and Downstream Inflammation-Related Pathway in Two Rat Nerve Injury Models [ eNeuro, 2023, 10(9)ENEURO.0185-23.2023] PubMed: 37620149
Nicotine Decreases Nerve Regeneration and Pain Behaviors via PTEN and Downstream Inflammation-Related Pathway in Two Rat Nerve Injury Models [ eNeuro, 2023, 10(9)ENEURO.0185-23.2023] PubMed: 37620149
PTEN/AKT signaling pathway related to hTERT downregulation and telomere shortening induced in Toxoplasma GRA16-expressing colorectal cancer cells [ Biomed Pharmacother, 2022, 153:113366] PubMed: 35810694
Formononetin inhibits IL-1β-induced inflammation in human chondrocytes and slows the progression of osteoarthritis in rat model via the regulation of PTEN/AKT/NF-κB pathway [ Int Immunopharmacol, 2022, 113(Pt A):109309] PubMed: 36306560
Exosomal miR-10b-5p mediates cell communication of gastric cancer cells and fibroblasts and facilitates cell proliferation [ J Cancer, 2021, 12(7):2140-2150] PubMed: 33754012
Assessment of Alveolar Macrophage Dysfunction Using an in vitro Model of Acute Respiratory Distress Syndrome [ Front Med (Lausanne), 2021, 8:737859] PubMed: 34660643
Three paralogous clusters of the miR-17~92 family of microRNAs restrain IL-12-mediated immune defense. [ Cell Mol Immunol, 2020, 10.1038/s41423-020-0363-5] PubMed: 32015501
MicroRNAs of the miR-17~9 family maintain adipose tissue macrophage homeostasis by sustaining IL-10 expression [ Elife, 2020, 9e55676] PubMed: 33150865
MicroRNAs of the miR-17~9 family maintain adipose tissue macrophage homeostasis by sustaining IL-10 expression [ Elife, 2020, 9e55676] PubMed: 33150865

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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