受注:045-509-1970 |
技術サポート:[email protected] 平日9:00〜18:00 1営業日以内にご連絡を差し上げます |
Synonyms | 2-fluorofucose | Storage (From the date of receipt) |
3 years -20°C powder | |
化学式 | C6H11FO4 |
|||
分子量 | 166.15 | CAS No. | 2089647-47-0 | |
Solubility (25°C)* | 体外 | DMSO | 33 mg/mL (198.61 mM) | |
Water | 33 mg/mL (198.61 mM) | |||
Ethanol | 8 mg/mL (48.14 mM) | |||
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. |
製品説明 | SGN‐2FF (2-fluorofucose) is an orally bioavailable small‐molecule inhibitor of fucosyltransferase that demonstrated encouraging preclinical antitumor activity in mouse models. |
---|---|
in vitro | SGN‐2FF can fully inhibit the fucosylation of the h1F6 mAb at the lowest screening concentration in CHO cells treated with SGN‐2FF. The functional effect of inhibiting fucosylation of LS174T cells with SGN‐2FF is apparent from its diminished adherence to E-selectin–coated plates.[2] |
in vivo | In mice, SGN‐2FF inhibits fucosylation of endogenously produced antibodies, tumor xenograft membranes, and neutrophil adhesion glycans. SGN‐2FF treatment affords complete protection from tumor engraftment in a syngeneic tumor vaccine model, inhibits neutrophil extravasation, and delays the outgrowth of tumor xenografts in immune-deficient mice. [2] |
細胞アッセイ | 細胞株 | human LS174T colorectal cells, CHO cells |
---|---|---|
濃度 | 100 μM | |
反応時間 | 10 days | |
実験の流れ | LS174T cells are cultured in Eagle MEM with 10% FBS with or without 100 μM SGN‐2FF for 10 d. LS174T xenografts from SGN‐2FF-treated or untreated mice are disaggregated by treatment with collagenase, and loose cells are collected. Cells from either source are washed with PBS solution containing 2% FBS and 0.02% sodium azide and stained with biotinylated LCA, cBR96-Alexa Fluor 488, or SSEAI–Alexa Fluor 488 (1 h on ice). Cells are washed and resuspended in PBS solution/FBS/sodium azide at 4 °C. The lectin-stained cells are treated with FITC-conjugated avidin D (30 min on ice), washed, and then analyzes by flow cytometry. |
|
動物実験 | 動物モデル | Mouse |
投薬量 | 1 mM, 10 mM, 100 mM | |
投与方法 | i.g. |
|
FUT8-Mediated Core Fucosylation Promotes the Pulmonary Vascular Remodeling in Pulmonary Arterial Hypertension [ Aging Dis, 2023, 14(5):1927-1944] | PubMed: 37196106 |
FUT8-Mediated Core Fucosylation Promotes the Pulmonary Vascular Remodeling in Pulmonary Arterial Hypertension [ Aging Dis, 2023, 14(5):1927-1944] | PubMed: 37196106 |
長期の保管のために-20°Cの下で製品を保ってください。
人間や獣医の診断であるか治療的な使用のためにでない。
各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。