SMER28

製品コードS8240 バッチS824002

化学情報

	Synonyms	N/A	Storage (From the date of receipt)	3 years -20°C powder 1 years -80°C in solvent
	化学式	C₁₁H₁₀BrN₃
	分子量	264.12	CAS No.	307538-42-7
Solubility (25°C)*	体外	DMSO	53 mg/mL (200.66 mM)
		Ethanol	53 mg/mL (200.66 mM)
		Water	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	SMER28 is a small-molecule enhancer (SMER) of autophagy, inducing autophagy independently of rapamycin in mammalian cells.
in vitro	SMER28 is a positive regulator of autophagy in a mechanism independent from the mTOR pathway. It increases autophagosome synthesis and enhances the clearance of model autophagy substrates such as A53T a-synuclein and mutant huntingtin fragments. A marked decrease in Aβ/APP-CTF levels and enhancement for degradation of Aβ/APP-CTF via autophagy is also induced by SMER28. APP-CTF and LC3-II are cocompartmentalized on SMER28 treatment^[1].

製品説明

SMER28 is a small-molecule enhancer (SMER) of autophagy, inducing autophagy independently of rapamycin in mammalian cells.

in vitro

SMER28 is a positive regulator of autophagy in a mechanism independent from the mTOR pathway. It increases autophagosome synthesis and enhances the clearance of model autophagy substrates such as A53T a-synuclein and mutant huntingtin fragments. A marked decrease in Aβ/APP-CTF levels and enhancement for degradation of Aβ/APP-CTF via autophagy is also induced by SMER28. APP-CTF and LC3-II are cocompartmentalized on SMER28 treatment^[1].

プロトコル(参考用のみ)

細胞アッセイ	細胞株	MEF cells overexpressing βCTF/ N2a-APP cells/ Rat primary neuronal cultures
	濃度	10μM in MEF cells/0-50μM in N2a-APP cells and rat primary neuronal cultures
	反応時間	16hrs
	実験の流れ	MEF cells overexpressing βCTF are treated with autophagy-enhancing compounds (10 μM) for 16 h. Cell lysates are analyzed by SDS-PAGE and Western blotting using anti-βCTF (6E10) and anti-tubulin antibodies.

参考

[1] Tian Y, et al. FASEB J. 2011, 25(6):1934-1942.

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Drug antagonism and single-agent dominance result from differences in death kinetics. [ Nat Chem Biol, 2020, 6]	PubMed: 32251407
Drug GRADE: An Integrated Analysis of Population Growth and Cell Death Reveals Drug-Specific and Cancer Subtype-Specific Response Profiles [ Cell Rep, 2020, 31(12):107800]	PubMed: 32579927
Stimulation of Hair Growth by Small Molecules that Activate Autophagy [ Cell Rep, 2019, 27(12):3413-3421]	PubMed: 31216464
Trehalose Modulates Autophagy Process to Counteract Gliadin Cytotoxicity in an In Vitro Celiac Disease Model. [ Cells, 2019, 8(4)]	PubMed: 31013754
Novel cell-and tissue-based assays for detecting misfolded and aggregated protein accumulation within aggresomes and inclusion bodies [Shen D Cell Biochem Biophys, 2011, 60(3):173-85]	PubMed: 21132543

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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