Sophoridine

製品コードS3895 バッチS389501

印刷

化学情報

Chemical Structure Synonyms 5-Epidihydrosophocarpine, Dihydro-5-episophocarpine Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

C15H24N2O
分子量 248.36 CAS No. 6882-68-4
Solubility (25°C)* 体外 DMSO 49 mg/mL (197.29 mM)
 
 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 Sophoridine (5-Epidihydrosophocarpine, Dihydro-5-episophocarpine), a natural product obtained from medicinal plants, has a variety of pharmacological effects, including anti-cancer and anti-arrhythmia, and affects the immune and central nervous systems.
in vitro Sophoridine kills cancer cells but has low cytotoxicity to normal cells. Pancreatic cancer cells are particularly sensitive. Sophoridine inhibits the proliferation of pancreatic cancer cells and induces cell cycle arrest at S phase and mitochondrial-related apoptosis. Moreover, Sophoridine induces a sustained activation of the phosphorylation of ERK and JNK. In addition, Sophoridine provokes the generation of reactive oxygen species (ROS) in pancreatic cancer cells[1]. Sophoridine also triggers significant down-regulated the expression of p27, CDK2, Survivin, Livin, Bcl-2, E2F1 and the transcriptional activity of FoxM1, NF-κb and AP-1, meanwhile, up-regulated the expression of caspase-3/8, p53, Smac, c-JNK and p38-MAPK. Sophoridine significantly inhibits ubiquitin-proteasome in tumor cells[2].
in vivo Sophoridine is an effective agent that can inhibit the growth of xenograft pancreatic tumors in vivo with well-tolerated toxicity[1]. Sophoridine attenuates intestinal inflammation in the experimental colitis model, which is associated with the downregulation of ICAM-1 gene expression[3].

プロトコル(参考用のみ)

細胞アッセイ 細胞株 Miapaca-2 and PANC-1; HPDE
濃度 0, 10, 20, 40, 80, or 100 μM
反応時間 24 h, 48 h, or 72 h
実験の流れ Two human pancreatic cancer cell lines (Miapaca-2 and PANC-1) and pancreatic ductal epithelial cell line (HPDE) are treated with Sophoridine at different concentrations (0, 10, 20, 40, 80, or 100 μM) for different lengths of time (24 h, 48 h, or 72 h). Cell viability is determined by the CCK-8 assay.
動物実験 動物モデル BALB/c homozygous (nu/nu) nude mice (6 weeks, 18 g)
投薬量 20 mg/kg or 40 mg/kg
投与方法 i.p.

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Sophoridine suppresses lenvatinib-resistant hepatocellular carcinoma growth by inhibiting RAS/MEK/ERK axis via decreasing VEGFR2 expression [ J Cell Mol Med, 2020, 10.1111/jcmm.16108] PubMed: 33210432

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。