SU1498

製品コードS6535 バッチS653501

化学情報

	Synonyms	N/A	Storage (From the date of receipt)	3 years -20°C powder
	化学式	C₂₅H₃₀N₂O₂
	分子量	390.52	CAS No.	168835-82-3
Solubility (25°C)*	体外	DMSO	78 mg/mL (199.73 mM)
		Ethanol	78 mg/mL (199.73 mM)
		Water	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	SU1498, a powerful inhibitor of KDR (IC50 = 0.7 μM), stimulates accumulation of phosphorylated ERK1/2 in endothelial cells.
in vitro	SU1498 accentuates accumulation of phosphorylated ERK in endothelial cells and inhibits its kinase activity while having no effect on phosphorylation of Akt. SU1498 does not affect phospho-ERK translocation to nucleus^[1].

プロトコル(参考用のみ)

細胞アッセイ	細胞株	HUVECs
	濃度	10 μM
	反応時間	15 min
	実験の流れ	Cells are inoculated into the wells of 6-well culture plates (3 × 10⁵ cells/well) and grown for 16 h in DMEM-based medium at 37 °C in the atmosphere of 5% CO2. After an overnight starvation (0.5% fetal bovine serum), the medium is removed, and the cells are washed with PBS and overlaid with 0.4 ml of DMEM. From this point on, all incubations are done at 25 °C. The inhibitors (SU1498, 10 μM; AG1433, 10 μM; AG1296, 25 μM; PD153035, 1 μM) are added and followed 15 min later by the growth factors: SPP, 1 μm; VEGF, 100 ng/ml; HGF, 100 ng/ml; bFGF, 10 ng/ml; EGF, 10 ng/ml. Forskolin (10 μm), a gratuitous inducer of adenylyl cyclase, is used as a control because of its known ability to enhance ERK phosphorylation. After a 10-min incubation, the medium is siphoned off, and the cells are lysed with 100 μl of lysis buffer. Samples (10 μl) of each lysate are fractionated by SDS-PAGE, and proteins are transferred to nitrocellulose and probed with anti-phospho-Akt or anti-phospho-ERK1/2 antibody. To show comparable loading, anti-actin antibody is used.

参考

[1] Boguslawski G, et al. J Biol Chem. 2004, 279(7):5716-24.

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Low-Dose Sorafenib Acts as a Mitochondrial Uncoupler and Ameliorates Nonalcoholic Steatohepatitis. [ Cell Metab, 2020, 5;31(5):892-908 e11]	PubMed: 32375062
[ Biol Open, 2018, ]	PubMed: 30232195

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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