受注:045-509-1970 |
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Synonyms | N/A | Storage (From the date of receipt) |
3 years -20°C powder 1 years -80°C in solvent |
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化学式 | C21H20N2OS3 |
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分子量 | 412.59 | CAS No. | 459147-39-8 | ||||||||
Solubility (25°C)* | 体外 | DMSO | 83 mg/mL (201.16 mM) | ||||||||
Ethanol (warmed with 50ºC water bath) | 5 mg/mL (12.11 mM) | ||||||||||
Water | Insoluble | ||||||||||
体内 (毎回新しく調製した物を用意してください) |
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* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. |
製品説明 | SW033291 is a selective inhibitor of 15-hydroxyprostaglandin dehydrogenase (15-PGDH) with IC50 and Ki of 1.5 nM and 0.1 nM, respectively. |
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in vitro | In Vaco-503 cells, SW033291 (2.5 μM) decreases cellular 15-PGDH enzyme activity by 85%.[1] |
in vivo | In mice receiving a bone marrow transplant, SW033291 (10 mg/kg, i.p.) promotes hematopoietic recovery. In mouse models of colon and liver injury, SW033291 (10 mg/kg, i.p.) reduces the levels of colitis-associated inflammatory cytokines, protects mice from colitis, and promotes liver regeneration. [1] |
キナーゼアッセイ | Activity assays of recombinant 15-PGDH protein | |
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For initial characterization of inhibition of 15-PGDH enzyme activity by SW033291, reactions are assembled with experiment specific concentrations of 15-PGDH enzyme, and experiment specific concentrations of SW033291, plus 150 μM NAD(+) and 25 μM PGE2 in reaction buffer (50 mM Tris-HCl, pH7.5, 0.01% Tween 20). The reaction mix is incubated for 15 min at 25 ºC in an Envision Reader. Enzyme activity is determined by following generation of NADH as assayed by recording fluorescence at Ex/Em=340 nM/485 nM every 30s for 3 minutes, commencing immediately after addition of PGE2. IC50 values are calculated with GraphPad Prism 5 software using the sigmoidal dose-response function and plotted against SW033291 concentration. | ||
細胞アッセイ | 細胞株 | BMSCs |
濃度 | 1 μM | |
反応時間 | 5 days | |
実験の流れ | Cells were treated with indicated concentration of drug for 5 days. |
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動物実験 | 動物モデル | Mice receiving a bone marrow transplant, and Mouse models of colon and liver injury |
投薬量 | 10 mg/kg twice daily | |
投与方法 | i.p. |
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Divalent metal cations stimulate skeleton interoception for new bone formation in mouse injury models [ Nat Commun, 2022, 13(1):535] | PubMed: 35087048 |
Skeleton interoception regulates bone and fat metabolism through hypothalamic neuroendocrine NPY [ Elife, 2021, 10e70324] | PubMed: 34468315 |
3D GelMA ICC Scaffolds Combined with SW033291 for Bone Regeneration by Modulating Macrophage Polarization [ Pharmaceutics, 2021, 13(11)1934] | PubMed: 34834349 |
Sensory nerves regulate mesenchymal stromal cell lineage commitment by tuning sympathetic tones. [ J Clin Invest, 2020, 131554] | PubMed: 32191640 |
Prostaglandin E2 mediates sensory nerve regulation of bone homeostasis. [ Nat Commun, 2019, 10(1):181] | PubMed: 30643142 |
長期の保管のために-20°Cの下で製品を保ってください。
人間や獣医の診断であるか治療的な使用のためにでない。
各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。