Tat-beclin 1 (Tat-BECN1)

製品コードS8595 バッチS859501

化学情報

	Synonyms	N/A	Storage (From the date of receipt)	3 years -20°C powder 1 years -80°C in solvent
	化学式	C₁₆₄H₂₅₁N₅₇O₄₅
	分子量	3741.1	CAS No.	1423821-88-8
Solubility (25°C)*	体外	Water	100 mg/mL (26.73 mM)


* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	Tat-beclin 1 (Tat-BECN1), a peptide known to stimulate autophagy through mobilization of endogenous Beclin 1, induces autophagy in vitro and in vivo and improves clinical outcomes.
in vitro	Tat-beclin 1 is a cell-permeable peptide that induces autophagy. Treatment with Tat-beclin 1, but not Tat-scrambled, results in a dose-dependent decrease in amounts of p62, a selective autophagy substrate, and a dose-dependent conversion of the non-lipidated form of LC3, LC3-I, to the lipidated, autophagosome-associated form of LC3, LC3-II, in multiple cell lines and primary murine embryonic fibroblasts (MEFs). Tat-beclin 1 markedly inhibits HIV-1 replication in primary human monocyte-derived macrophages^[1].
in vivo	Tat-beclin 1 can induce autophagy in peripheral tissues in adult mice as well as in the central nervous system of neonatal mice. Daily Tat-beclin 1 peptide treatment is well-tolerated in both neonatal and adult mice. Tat-beclin 1 peptide improves the clinical outcome of mice with CNS WNV(West Nile virus) infection^[1].

プロトコル(参考用のみ)

細胞アッセイ	細胞株	human MDMs
	濃度	0.5–5 μM
	反応時間	24 h
	実験の流れ	For treatment of primary human MDMs, cells are washed with PBS and pre-treated with peptides (0.5-5 μM, 24 h) in 500 μl macrophage-SFM (serum-free media) before infection with HIV-1.
動物実験	動物モデル	C57BL/6J mice
	投薬量	15 mg/kg
	投与方法	i.p.

参考

[1] Shoji-Kawata S, et al. Nature. 2013, 494(7436):201-6.

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Chronic hypoxia stabilizes 3βHSD1 via autophagy suppression [ Cell Rep, 2024, 43(1):113575]	PubMed: 38181788
Small cytosolic double-stranded DNA represses cyclic GMP-AMP synthase activation and induces autophagy [ Cell Rep, 2023, 42(8):112852]	PubMed: 37481718
ASPP2 promotes cell apoptosis in cervical cancer through inhibiting autophagy [ Exp Ther Med, 2022, 24(6):726]	PubMed: 36340606
Autophagy induction during stem cell activation plays a key role in salivary gland self-renewal [ Autophagy, 2021, 1-16]	PubMed: 34009100
Moderately Inducing Autophagy Reduces Tertiary Brain Injury after Perinatal Hypoxia-Ischemia [ Cells, 2021, 10(4)898]	PubMed: 33919804
Sirt7 protects chondrocytes degeneration in osteoarthritis via autophagy activation [ Eur Rev Med Pharmacol Sci, 2020, 24(18):9246-9255]	PubMed: 33015765
Impaired Vps34 complex activity-mediated autophagy inhibition contributes to endothelial progenitor cells damage in the ischemic conditions. [ Biochem Biophys Res Commun, 2020, 524(3):629-635]	PubMed: 32029275
Glycolytic Enzyme PKM2 Mediates Autophagic Activation to Promote Cell Survival in NPM1-Mutated Leukemia. [ Int J Biol Sci, 2019, 15(4):882-894]	PubMed: 30906218
Autophagy facilitates the release of immunogenic signals following chemotherapy in 3D models of mesothelioma. [ Mol Carcinog, 2019, 10.1002/mc.23050]	PubMed: 31215708

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。