Tauroursodeoxycholic Acid (TUDCA)

製品コードS3654 バッチS365406

化学情報

	Synonyms	N/A	Storage (From the date of receipt)	3 years -20°C powder 1 years -80°C in solvent
	化学式	C₂₆H₄₅NO₆S
	分子量	499.70	CAS No.	14605-22-2
Solubility (25°C)*	体外	DMSO	100 mg/mL (200.12 mM)
		Water	100 mg/mL (200.12 mM)
		Ethanol	50 mg/mL (100.06 mM)
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	Tauroursodeoxycholic acid (TUDCA) is the taurine conjugate of ursodeoxycholic acid (UDCA) that acts as a mitochondrial stabilizer and anti-apoptotic agent in several models of neurodegenerative diseases, including AD, Parkinson's diseases (PD), and Huntington's diseases (HD).
in vitro	Tauroursodeoxycholic acid (TUDCA) is an endogenous hydrophilic tertiary bile acid produces in humans at a low level. In ER stress conditions, TUDCA treatment of MSCs (mesenchymal stem cells) reduces the activation of ER stress-associated proteins, including GRP78, PERK, eIF2α, ATF4, IRE1α, JNK, p38, and CHOP, and inhibits the dissociation between GRP78 and PERK, resulting in reduced ER stress-mediated cell death. TUDCA treatment increases PrP^C (Cellular prion protein) expression. TUDCA regulates stem cell differentiation into various lineages such as adipogenic and osteogenic lineages. TUDCA attenuates ER stress, prevents unfolded protein response dysfunction, and stabilizes mitochondria. Under ER stress, treatment with TUDCA significantly increases the expression of BCL-2 and significantly decreases the expression of Bax, cleaved caspase-3, and cleaved PARP-1, compared with that of untreated cells^[1].
in vivo	TUDCA is effective for treating cholestatic liver diseases. It also has an ameliorating effect on several diseases, including neurodegenerative diseases, osteoarthritis, vascular diseases, and diabetes. In a murine hindlimb ischemia model, TUDCA-treated mesenchymal stem cells (MSCs) transplantation augments the blood perfusion ratio, vessel formation, and transplanted cell survival more than untreated MSC transplantation does. Augmented functional recovery following MSC transplantation is blocked by PrP^C downregulation^[1]. Several studies in animals have shown that TUDCA, an endogenous ambiphilic bile acid, can inhibit unfolded protein response dysfunction and ameliorate ER stress. TUDCA administration attenuates HDM-induced ER stress, airway inflammation, mucus metaplasia, airway remodeling, and methacholine-induced AHR^[2].

プロトコル(参考用のみ)

細胞アッセイ	細胞株	Mesenchymal stem cells (MSCs)
	濃度	100 μM
	反応時間	30 min
	実験の流れ	MSCs are washed twice with phosphate buffer saline (PBS), and fresh α-MEM supplemented with 10% FBS is added. To investigate the apoptosis signaling pathway, MSCs are pretreated with TUDCA (100 μM) at 37 °C for 30 min and then treated with H2O2 (200 μM) for various times (0, 2, 4, 6, or 8 h). To assess another cell signaling pathway, MSCs are treated with an Akt inhibitor (10⁻⁶ M) for 30 min at 37 °C before treatment with TUDCA.
動物実験	動物モデル	House dust mite-induced allergic airway disease mouse model (background: C57BL/6 NJ mice)
	投薬量	0.5, 1, and 5 mg/kg body wt
	投与方法	via the nasopharynx

参考

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Advanced oxidation protein products induce Paneth cells defects by endoplasmic reticulum stress in Crohn's disease [ iScience, 2023, 26(8):107312]	PubMed: 37539032
Routes of Albumin Overload Toxicity in Renal Tubular Epithelial Cells [ Int J Mol Sci, 2023, 24(11)9640]	PubMed: 37298591
Tauroursodeoxycholic Acid Supplementation in In Vitro Culture of Indicine Bovine Embryos: Molecular and Cellular Effects on the In Vitro Cryotolerance [ Int J Mol Sci, 2023, 24(18)14060]	PubMed: 37762363
Tauroursodeoxycholic acid protects Schwann cells from high glucose-induced cytotoxicity by targeting NLRP3 to regulate cell migration and pyroptosis [ Biotechnol Appl Biochem, 2023, 10.1002/bab.2518]	PubMed: 37749820
Very-low-density lipoprotein receptor-enhanced lipid metabolism in pancreatic stellate cells promotes pancreatic fibrosis [ Immunity, 2022, 55(7):1185-1199.e8]	PubMed: 35738281
Mitochondrial fission links ECM mechanotransduction to metabolic redox homeostasis and metastatic chemotherapy resistance [ Nat Cell Biol, 2022, 24(2):168-180]	PubMed: 35165418
Tauroursodeoxycholic acid functions as a critical effector mediating insulin sensitization of metformin in obese mice [ Redox Biol, 2022, 57:102481]	PubMed: 36148770
Tripartite motif 25 ameliorates doxorubicin-induced cardiotoxicity by degrading p85α [ Cell Death Dis, 2022, 13(7):643]	PubMed: 35871160
Endoplasmic reticulum stress contributes to cisplatin-induced chronic kidney disease via the PERK-PKCδ pathway [ Cell Mol Life Sci, 2022, 79(8):452]	PubMed: 35895146
S1PR2/RhoA/ROCK1 pathway promotes inflammatory bowel disease by inducing intestinal vascular endothelial barrier damage and M1 macrophage polarization [ Biochem Pharmacol, 2022, 201:115077]	PubMed: 35537530

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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