受注:045-509-1970 |
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Synonyms | NP 01139 | Storage (From the date of receipt) |
3 years -20°C powder 1 years -80°C in solvent |
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化学式 | C10H10N2O2S |
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分子量 | 222.26 | CAS No. | 327036-89-5 | |
Solubility (25°C)* | 体外 | DMSO | 44.5 mg/mL (200.21 mM) | |
Ethanol | 44.5 mg/mL (200.21 mM) | |||
Water | Insoluble | |||
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. |
製品説明 | TDZD-8 (NP 01139) is a non-ATP competitive GSK-3β inhibitor with IC50 of 2 μM; minimal inhibitory effect observed on CDK1, casein kinase II, PKA and PKC. |
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in vitro | TDZD-8 acts as a noncompetitive inhibitor of ATP or GS-1 binding. TDZD-8 shows no inhibition of PKA, PKC, Cdk-1/cyclin B or CK-II in kinase assays. [1] TDZD-8 specifically induces cell death of primary leukemia specimens. TDZD-8 ablates leukemia progenitor and stem cells. TDZD-8 treatment induces oxidative stress. TDZD-8 induces cell death with extremely rapid cell death kinetics showing loss of membrane integrity. TDZD-8 inhibits PKC and FLT3 in primary AML specimens. [2] |
キナーゼアッセイ | GSK-3β inhibition | |
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GSK-3β enzyme isincubated with 15 μM of ATP, 0.2μCi of [γ- 32 P]ATP, GS-1 as substrate and different concentrations of the test compound. GSK-3 activity isassayed in 50 mM Tris-HCl, pH 7.5, 10 mM MgCl2, 1 mM EGTA, and 1 mM EDTA buffer, at 37 ℃, in the presence of 15 μM GS-1 (substrate), 15 μM [γ-32P]ATP in a final volume of 12 μL. After 20 min incubation at 37 ℃, 4 μL aliquots of the supernatant are spotted onto 2 × 2 cm pieces of Whatman P81 phosphocellulose paper, and 20s later, the filters are washed four times (for at least 10 min each time) in 1% phosphoric acid. The dried filters are transferred into scintillation vials, and the radioactivity ismeasured in a liquid scintillation counter. Blank values are subtracted, and the GSK-3β activity isexpressed in picomoles of phosphate incorporated in GS-1 per 20 min or in percentage of maximal activity. |
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Data from [Data independently produced by , , Int Immunopharmacol. 2017, 43:164-171.]
β-Catenin and SOX2 Interaction Regulate Visual Experience-Dependent Cell Homeostasis in the Developing Xenopus Thalamus [ Int J Mol Sci, 2023, 24(17)13593] | PubMed: 37686400 |
GSK-3β inhibition alleviates arthritis pain via reducing spinal mitochondrial reactive oxygen species level and inflammation [ PLoS One, 2023, 18(4):e0284332] | PubMed: 37058473 |
Glycogen synthase kinase-3β inhibition decreases inflammation and relieves cancer induced bone pain via reducing Drp1-mediated mitochondrial damage [ J Cell Mol Med, 2022, 26(14):3965-3976] | PubMed: 35689386 |
The VRK1 chromatin kinase regulates the acetyltransferase activity of Tip60/KAT5 by sequential phosphorylations in response to DNA damage [ Biochim Biophys Acta Gene Regul Mech, 2022, 1865(8):194887] | PubMed: 36280132 |
Activation of NRF2 ameliorates oxidative stress and cystogenesis in autosomal dominant polycystic kidney disease [ Sci Transl Med, 2020, 12(554):eaba3613] | PubMed: 32727915 |
AMPK/GSK3β/β-catenin cascade–triggered overexpression of CEMIP promotes migration and invasion in anoikis-resistant prostate cancer cells by enhancing metabolic reprogramming [Zhang P FASEB J, 2018, 32(7):3924-3935] | PubMed: 29505302 |
Identifying Host Regulators and Inhibitors of Liver Stage Malaria Infection Using Kinase Activity Profiles [ Nat Commun, 2017, 1;8(1):1232] | PubMed: 29089541 |
Purinergic P2X7 receptor mediates acetaldehyde-induced hepatic stellate cells activation via PKC-dependent GSK3β pathway [Wu X Int Immunopharmacol, 2017, 43:164-171] | PubMed: 28061416 |
長期の保管のために-20°Cの下で製品を保ってください。
人間や獣医の診断であるか治療的な使用のためにでない。
各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。