Thymopentin

製品コードS3668 バッチS366801

印刷

化学情報

Chemical Structure Synonyms TP5 Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

C30H49N9O9
分子量 679.77 CAS No. 69558-55-0
Solubility (25°C)* 体外 DMSO 100 mg/mL (147.1 mM)
Water 100 mg/mL (147.1 mM)
Ethanol Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 Thymopentin (TP5) has immuno-regulatory activities. The immuno-regulatory actions of thymopentin on peripheral T cells are mediated by intracellular cyclic GMP elevations in contrast to the intracellular cyclic AMP elevations induced in precursor T cells that trigger their further differentiation to T cells.
in vitro TP5 displays concentration-dependent inhibitory effects on the proliferation and colony formation of HL-60 cells. the decrease or even disappearance of AgNORs from nucleoli is observed in HL-60 cells after the treatment with TP5. The suppression induced by TP5 is accompanied by an accumulation of cell cycle in the G0/G1 phase. Moreover, TP5 significantly increases the NBT-reduction activity of HL-60 cells. TP5 has induced differentiation along the granulocytes lineage in HL-60 cells[1]. TP5 increases the expression of CD4 and CD8 markers on the surface of human thymocytes.
in vivo TP5 is able to markedly stimulate the activity of immune system cells in healthy animals. It activates the production of a number of cytokines (IL1α, IL2, IL6, IL10, IFNγ), NO, Hsp70 and Hsp90 in mature cells of the peripheral immune system of mice (peritoneal macrophages and spleen lymphocytes)[2].

プロトコル(参考用のみ)

細胞アッセイ 細胞株 HL-60 cells
濃度 100–800 μmol/L
反応時間 120 h
実験の流れ HL-60 cells are plated at a density of 1 × 105 cells/mL, and cultured for 120h in a medium containing TP5 at various concentrations (100-800 μmol/L). To quantify the number of cells after treatment, cells are counted at different time points of incubation using a hemocytometer under a light microscope. Cell viability is determined by the Trypan blue exclusion assay. Viability is calculated as the ratio of living cells to the total cell population.
動物実験 動物モデル NMRI mice
投薬量 15 µg/100 g
投与方法 i.p.

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Novel Natural Inhibitors Targeting Enhancer of Zeste Homolog 2: A Comprehensive Structural Biology Research [ Front Oncol, 2021, 11:741403] PubMed: 34737956

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。