受注:045-509-1970 |
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Synonyms | N/A | Storage (From the date of receipt) |
3 years -20°C powder | |
化学式 | C26H24IN7O |
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分子量 | 577.42 | CAS No. | 351986-85-1 | |
Solubility (25°C)* | 体外 | DMSO | 10 mg/mL (17.31 mM) | |
Water | Insoluble | |||
Ethanol | Insoluble | |||
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. |
製品説明 | Vacuolin-1 is a potent and cell-permeable inhibitor that blocks the Ca(2+)-dependent exocytosis of lysosomes and prevents the release of lysosomal content without affecting the process of resealing. Vacuolin-1 is also a potent and selective PIKfyve inhibitor, and inhibits autophagy by impairing lysosomal maturation via PIKfyve inhibition. |
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in vitro | Vacuolin-1 blocks both ionophore- and wound-induced, Ca2+-dependent exocytosis of lysosomes, but does not block Ca2+-dependent fusion of enlargeosomes with the plasma membrane.[1] |
in vivo | Vacuolin-1 significantly suppresses the metastasis and/or tumor growth of breast cancer or melanoma. Vacuolin-1 targets Capping protein Zβ (CapZβ) to inhibit endosomal trafficking and metastasis.[3] |
細胞アッセイ | 細胞株 | Human HeLa cells, Human A431 cells, monkey BSC-1 cells |
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濃度 | 1 μM-10 μM | |
反応時間 | 20-180 min | |
実験の流れ | HeLa cells grown on 25 mm diameter coverslips in six-well plates are incubated for 10 min with BSA conjugated with 5 nm gold particles, followed by a 3–12 h incubation in a medium without BSA–gold. During the last 20–180 min of the chase, the cells are incubated in 0.2% DMSO with or without 1 μM vacuolin-1. For electron microscopy, cells are washed with PBS, fixed at room temperature for 30 min, and processed for ultrathin cryosectioning and immunolabelling. |
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動物実験 | 動物モデル | MMTV-PyMT transgenic mouse model, orthotopic metastatic breast cancer mouse model |
投薬量 | 10 mg/kg | |
投与方法 | i.p |
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Induction of lysosomal exocytosis and biogenesis via TRPML1 activation for the treatment of uranium-induced nephrotoxicity [ Nat Commun, 2023, 14(1):3997] | PubMed: 37414766 |
Induction of lysosomal exocytosis and biogenesis via TRPML1 activation for the treatment of uranium-induced nephrotoxicity [ Nat Commun, 2023, 14(1):3997] | PubMed: 37414766 |
Endocytosis, Distribution, and Exocytosis of Polystyrene Nanoparticles in Human Lung Cells [ Nanomaterials (Basel), 2022, 13(1)84] | PubMed: 36615994 |
長期の保管のために-20°Cの下で製品を保ってください。
人間や獣医の診断であるか治療的な使用のためにでない。
各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。