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Synonyms | N/A | Storage (From the date of receipt) |
3 years -20°C powder | |
化学式 | C17H15N7 |
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分子量 | 317.35 | CAS No. | 327031-55-0 | |
Solubility (25°C)* | 体外 | DMSO | 13 mg/mL (40.96 mM) | |
Water | ˂1 mg/mL | |||
Ethanol | ˂1 mg/mL | |||
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. |
製品説明 | VLX600 is a novel iron-chelating inhibitor of oxidative phosphorylation (OXPHOS), potentiates the effect of radiation in tumor spheroids in a synergistic manner. VLX600 shows enhanced cytotoxic activity under conditions of nutrient starvation. VLX600 induces autophagy and mitochondrial inhibition with antitumor activity. |
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in vitro | VLX600 shows enhanced cytotoxic activity under conditions of nutrient starvation.[1] Mitochondrial inhibition with VLX600 has a direct antitumor effect in vitro while appearing to promote glycolysis through increased AKT signaling and glucose transporter expression. When combined with imatinib, VLX600 prevents imatinib-induced cell cycle escape and reduces p27 expression, leading to increased apoptosis when compared to imatinib alone.[3] |
in vivo | VLX600 induces the formation of autolysosomes in vivo. VLX600 displays tumour growth inhibition in vivo.[1] When combined with imatinib, VLX600 reduces p27 expression in vivo, and prevents imatinib-induced cell cycle escape, likely potentiating the antitumoral effects of Kit inhibition.[3] |
細胞アッセイ | 細胞株 | human GIST-T1 cell line, human HG129 cell line, human GIST-882 cell line, |
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濃度 | 2 μM, 6 μM, 12 μM | |
反応時間 | 48–72h | |
実験の流れ | To measure cell viability, 1 × 104 GIST-T1, GIST-882, and HG129 cells are seeded in replicates of 5 in a 96-well flat-bottomed plate (Falcon) and cultured for 48–72h with VLX600 or DMSO-only solvent controls. |
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動物実験 | 動物モデル | female NMRI nu/nu mice (8–10 weeks old) |
投薬量 | 16 mg/kg | |
投与方法 | IV |
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Iron Chelator VLX600 Inhibits Mitochondrial Respiration and Promotes Sensitization of Neuroblastoma Cells in Nutrition-Restricted Conditions [ Cancers (Basel), 2022, 14(13)3225] | PubMed: 35805002 |
長期の保管のために-20°Cの下で製品を保ってください。
人間や獣医の診断であるか治療的な使用のためにでない。
各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。