WZ811

製品コードS2912 バッチS291201

印刷

化学情報

Chemical Structure Synonyms N/A Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

 

C18H18N4
 
分子量 290.36 CAS No. 55778-02-4
Solubility (25°C)* 体外 DMSO 30 mg/mL (103.32 mM)
Water Insoluble
Ethanol Insoluble
体内 （毎回新しく調製した物を用意してください）
Clear solution
5%DMSO 40%PEG300 5%Tween80 50%ddH2O
1.5mg/ml Taking the 1 mL working solution as an example, add 50 μL of 30 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 WZ811 is a highly potent competitive CXCR4 antagonist with EC50 of 0.3 nM.
in vitro WZ811 is a highly potent competitive antagonist of CXCR4. WZ811 is effective in counteracting SDF-1 on cAMP reduction at doses as low as a few nanomoles. WZ811 blocks SDF-1-mediated invasion with EC50 of 5.2 nM. [1]

プロトコル(参考用のみ)

キナーゼアッセイ Binding affinity
For compound screening based on a competition binding assay, 2 × 104 MDA-MB-231 cells in 200μL of medium are seeded in an 8-well slide chamber 2 days before the experiments. Various concentrations of WZ811 (1, 10, 100, and 1000 nM) are added to the separate wells and incubated for 10 min at room temperature, and then the cells are fixed in 4% ice-cold paraformaldehyde. The cells are rehydrated in phosphate-buffered saline (PBS) and blocked to eliminate nonspecific binding. The slides are subsequently incubated for 45 min at room temperature with 0.05 μg/mL biotinylated 3, washed three times with PBS, and incubated in streptavidin-rhodamine (1:150 dilution) for 30 min at room temperature. Finally, the slides are washed with PBS and mounted in an antifade mounting solution.

カスタマーフィードバック

, , Invest New Drugs, 2017, 35(6):718-732

Data independently produced by , , Dr.Milica Pesic from Institute for Biological Research

Data independently produced by , , Dr. Milica Pesic from Institute for Biological Research

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Metformin potentiates nephrotoxicity by promoting NETosis in response to renal ferroptosis [ Cell Discov, 2023, 9(1):104] PubMed: 37848438
Metformin potentiates nephrotoxicity by promoting NETosis in response to renal ferroptosis [ Cell Discov, 2023, 9(1):104] PubMed: 37848438
Berbamine targets cancer stem cells and reverses cabazitaxel resistance via inhibiting IGF2BP1 and p-STAT3 in prostate cancer [ Prostate, 2023, 10.1002/pros.24632] PubMed: 37828768
The CXCR4-CXCL12 axis promotes T-cell reconstitution via efficient hematopoietic immigration [ J Genet Genomics, 2022, S1673-8527(22)00125-4] PubMed: 35483564
Single-cell transcriptomic reveals molecular diversity and developmental heterogeneity of human stem cell-derived oligodendrocyte lineage cells [ Nat Commun, 2021, 12(1):652] PubMed: 33510160
TGFβR-SMAD3 Signaling Induces Resistance to PARP Inhibitors in the Bone Marrow Microenvironment [ Cell Rep, 2020, 33(1):108221] PubMed: 33027668
Megakaryocytes participate in the occurrence of bleomycin-induced pulmonary fibrosis [ Cell Death Dis, 2019, 10(9):648] PubMed: 31501415
CXCR7 signaling promotes breast cancer survival in response to mesenchymal stromal stem cell-derived factors [ Cell Death Discov, 2019, 5:87] PubMed: 30993013
CXCR4-mediated signaling regulates autophagy and influences acute myeloid leukemia cell survival and drug resistance [Hu X Cancer Lett, 2018, 425:1-12] PubMed: 29574276
NMDA receptor activation inhibits the antifibrotic effect of BM-MSCs on bleomycin-induced pulmonary fibrosis [Li X Am J Physiol Lung Cell Mol Physiol, 2018, 315(3):L404-L421] PubMed: 29722562

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。