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Synonyms | (1S)-Z-FA-FMK | Storage (From the date of receipt) |
3 years -20°C powder 1 years -80°C in solvent |
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化学式 | C21 H23 F N2 O4 |
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分子量 | 386.42 | CAS No. | 197855-65-5 | |
Solubility (25°C)* | 体外 | DMSO | 77 mg/mL (199.26 mM) | |
Ethanol | 34 mg/mL warmed with 50ºC water bath (87.98 mM) | |||
Water | Insoluble | |||
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. |
製品説明 | Z-FA-FMK ((1S)-Z-FA-FMK) is an irreversible cysteine protease inhibitor, and also inhibits effector caspases. |
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in vitro | Z-FA-FMK inhibits the degradation of fibrillar collagen by fibroblasts and osteoclasts. [2] Z-FA-FMK inhibits LPS-induced cytokine production via inhibition of NF-kappaB-dependent gene expression in macrophages. [3] Z-FA-FMK efficiently blocks T cell proliferation induced by mitogens and IL-2 in vitro. [4] |
in vivo | Z-FA-FMK significantly increases pneumococcal growth in both lungs and blood in a mouse model of intranasal pneumococcal infection. [4] Z-FA-FMK blocks reovirus infection of Ras oncogenic tumours and host heart tissues in severe combined immunodeficiency mice. [5] |
細胞アッセイ | 細胞株 | PBMCs |
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濃度 | ~100 μM | |
反応時間 | 72 hours | |
実験の流れ | T cell proliferation following mitogen stimulation is determined using [3H]thymidine incorporation. In brief, PBMCs or purified T cells are seeded in a 96-well plate and stimulated with either PHA (5 μg/ml), costimulated with anti-CD3 mAb (5 μg/ml) and anti-CD28 mAb (2.5 μg/ml) or PMA plus ionomycin in the presence or absence of z-FA-FMK. The cells are cultured for 72 h with the last 16 h pulsed with [methyl-3H]thymidine (0.037 MBq). The cells are harvested onto glass fiber filter mats using a Tomtec automated multiwell harvester. | |
動物実験 | 動物モデル | Mouse model of pneumococcal disease |
投薬量 | ~25 mg/kg | |
投与方法 | i.v. |
Pharmacological targeting of TFIIH suppresses KRAS mutant pancreatic ductal adenocarcinoma and synergizes with TRAIL [ Cancer Res, 2022, can.21.4222] | PubMed: 35819261 |
Potential drug discovery for COVID-19 treatment targeting Cathepsin L using a deep learning-based strategy [ Comput Struct Biotechnol J, 2022, 20:2442-2454] | PubMed: 35602976 |
Targeting multiple cell death pathways extends the shelf life and preserves the function of human and mouse neutrophils for transfusion [ Sci Transl Med, 2021, 13(604)eabb1069] | PubMed: 34321317 |
Design of modular autoproteolytic gene switches responsive to anti-coronavirus drug candidates [ Nat Commun, 2021, 12(1):6786] | PubMed: 34811361 |
SARS-CoV-2 requires cholesterol for viral entry and pathological syncytia formation [ Elife, 2021, 10e65962] | PubMed: 33890572 |
Development of a Cell-Based Luciferase Complementation Assay for Identification of SARS-CoV-2 3CLpro Inhibitors [ Viruses, 2021, 13(2)173] | PubMed: 33498923 |
Autophagy Participates in Lysosomal Vacuolation-Mediated Cell Death in RGNNV-Infected Cells [ Front Microbiol, 2020, 30;11:790] | PubMed: 32425913 |
Ferroptosis Is Involved in Alcohol-Induced Cell Death in vivo and in vitro [ Biosci Biotechnol Biochem, 2020, 18;1-8] | PubMed: 32419644 |
[ Oncogene, 2018, ] | PubMed: 29326439 |
The Tumor Suppressor p53 Limits Ferroptosis by Blocking DPP4 Activity [ Cell Rep, 2017, 20(7):1692-1704] | PubMed: 28813679 |
長期の保管のために-20°Cの下で製品を保ってください。
人間や獣医の診断であるか治療的な使用のためにでない。
各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。