ZCL278

製品コードS7293 バッチS729301

印刷

化学情報

Chemical Structure Synonyms N/A Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

C21H19BrClN5O4S2
分子量 584.89 CAS No. 587841-73-4
Solubility (25°C)* 体外 DMSO 100 mg/mL (170.97 mM)
Water Insoluble
Ethanol Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 ZCL278 is a selective Cdc42 GTPase inhibitor with Kd of 11.4 μM.
in vitro ZCL278 inhibits Cdc42 GTPase activity by the competition with GTP and inhibits Rac/Cdc42 phosphorylation in a time-dependent manner. ZCL278 (50 μM) inhibits Cdc42-mediated microspike formation and disrupts GM130-docked Golgi structures in serum-starved Swiss 3T3 fibroblasts. In addition, ZCL278 also suppresses Cdc42-mediated neuronal branching and growth cone dynamics as well as actin-based motility and migration in a metastatic prostate cancer PC-3 cell line without cytotoxicity. [1]

プロトコル(参考用のみ)

キナーゼアッセイ p50RhoGAP or Cdc42GAP Assay
Inorganic phosphate produced as a result of GTPase activity is measured by using a p50RhoGAP or Cdc42GAP assay (Cytoskeleton) and absorbance-based detection method. Briefly, Cdc42 is preloaded with GTP or ZCL278 and incubated in the reaction buffer (provided in the kit) for 20 min at 37 °C. GAP is then added for an additional 20 min at 37 °C. Following a 10-min incubation in CytoPhos reagent (Cytoskeleton), inorganic phosphate was detected at 650 nm.

カスタマーフィードバック

, , Cell Physiol Biochem, 2015, 36(4):1613-27.

Data from [Data independently produced by , , J Bone Miner Res, 2018, 33(5):945-958]

Data from [Data independently produced by , , J Virol, 2017, 91(5)]

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Glutamine metabolic microenvironment drives M2 macrophage polarization to mediate trastuzumab resistance in HER2-positive gastric cancer [ Cancer Commun (Lond), 2023, 43(8):909-937] PubMed: 37434399
ARHGEF37 overexpression promotes extravasation and metastasis of hepatocellular carcinoma via directly activating Cdc42 [ J Exp Clin Cancer Res, 2022, 41(1):230] PubMed: 35869555
GEFT Inhibits Autophagy and Apoptosis in Rhabdomyosarcoma via Activation of the Rac1/Cdc42-mTOR Signaling Pathway [ Front Oncol, 2021, 11:656608] PubMed: 34221974
NecroX-5 Can Suppress Melanoma Metastasis by Reducing the Expression of Rho-Family GTPases [ J Clin Med, 2021, 10(13)2790] PubMed: 34201921
Epigenetically upregulated GEFT-derived invasion and metastasis of rhabdomyosarcoma via epithelial mesenchymal transition promoted by the Rac1/Cdc42-PAK signalling pathway. [ EBioMedicine, 2019, 50:122-134] PubMed: 31761617
Cdc42 Is Essential for Both Articular Cartilage Degeneration and Subchondral Bone Deterioration in Experimental Osteoarthritis [Hu X J Bone Miner Res, 2018, 33(5):945-958] PubMed: 29314205
Intersectin-Cdc42 interaction is required for orderly meiosis in porcine oocytes [Li X J Cell Physiol, 2018, 10.1002/jcp.27510] PubMed: 30478952
CDC42 controls the activation of primordial follicles by regulating PI3K signaling in mouse oocytes [ BMC Biol, 2018, 16(1):73] PubMed: 29976179
Interaction of the Small GTPase Cdc42 with Arginine Kinase Restricts White Spot Syndrome Virus in Shrimp [Xu JD J Virol, 2017, 91(5)] PubMed: 28031362
Exosomal miR-141-3p regulates osteoblast activity to promote the osteoblastic metastasis of prostate cancer [ Oncotarget, 2017, 8(55):94834-94849] PubMed: 29212270

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。