Zearalenone

製品コードS5676 バッチS567601

印刷

化学情報

Chemical Structure Synonyms F2 toxin, RAL Storage
(From the date of receipt)		 3 years -20°C powder
化学式

C18H22O5
分子量 318.36 CAS No. 17924-92-4
Solubility (25°C)* 体外 DMSO 64 mg/mL (201.03 mM)
Ethanol 64 mg/mL (201.03 mM)
Water Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 Zearalenone (F2 toxin, RAL) is a non-steroidal estrogenic mycotoxin that acts by binding the estrogen receptor (ER).
in vitro Zearalenone is able to occupy the active site of ERs in a strikingly similar manner as E2[1]. Zearalenone disrupts genome stability and inhibits growth of porcine granulosa cells via the estrogen receptors which may promote granulosa cell apoptosis when the DNA repair system is not enough to rescue this serious damage[3]. At low concentrations, ZEN enhances cell proliferation, increases colony formation and fastens cell migration after wound healing. Zearalenone exhibits carcinogenesis-like properties[4].
in vivo In ovariectomized female ICR mice, s.c. administration of ZEN at doses ≥2 mg/kg/day for 3 consecutive days significantly increased uterine wet weight compared with the control group[1]. Zearalenone is an immunotoxic compound similar to estrogen and some endocrine disruptors. After oral exposure, ZEA is rapidly absorbed and initially metabolized by the intestinal tissue and hepatocytes; this initiates the biotransformation of the compound into its major biologically active reductive metabolites, α- and β-zearalenol (α- and β-ZOL). The estrogenic activity of ZEA and its metabolites is mediated by their binding affinity to estrogen receptors (ER), and they are as potent as coumestrol and genistein, two endocrine-disrupting phytestrogens[2].

プロトコル(参考用のみ)

細胞アッセイ 細胞株 HCT116 cells
濃度 0-320 μM
反応時間 48 h
実験の流れ

Human colon carcinoma cells (HCT116) were maintained as monolayer culture in DMEM, supplemented with 10 % fetal bovine serum (FBS), 1 % l-glutamine (200 mM), 1 % of mixture penicillin (100 IU/ml) and streptomycin (100 μg/ml), in a humidified incubator at 37°C in an atmosphere of 5 % CO2 in air. ZEN is dissolved in pure ethanol. Cell viability was assessed using the methylene blue staining assay. Approximately 20,000 cells per well were seeded in 96-well plates with increasing ZEN or AFB1 concentrations (0-320 μM) at 37°C for 48 h in a final volume of 200 μl. The medium was then removed and cells were gently washed twice with PBS (1x), fixed with 3 % PFA for 5 min at room temperature then stained for 5 min with 0.1 % methylene blue. After extensive washing in water, methylene blue was eluted from cells using 0.1 % HCl, and the absorption of the supernatants was measured at 590 nm in a fluorimiter plate reader.
動物実験 動物モデル ovariectomized ICR mice
投薬量 0.5-100 mg/kg/day
投与方法 s.c.

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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