(20S)-Protopanaxadiol

別名:20-Epiprotopanaxadiol

20(S)-Protopanaxadiol (PPD, 20-Epiprotopanaxadiol), the main intestinal metabolite of ginsenosides, is one of the active ingredients in ginseng. 20(S)-Protopanaxadiol inhibits P-glycoprotein in multidrug resistant cancer cells.

(20S)-Protopanaxadiol化学構造

CAS No. 30636-90-9

サイズ 価格(税別) 在庫状況
JPY 18100 国内在庫あり
JPY 67900 国内在庫あり

代表番号: 045-509-1970|電子メール:[email protected]
よく尋ねられる質問

製品安全説明書

現在のバッチを見る: 純度: 99.96%
99.96

(20S)-Protopanaxadiol関連製品

シグナル伝達経路

P-gp阻害剤の選択性比較

Cell Data

Cell Lines Assay Type Concentration Incubation Time 活性情報 PMID
U87MG cells Function assay 43.4 μM 4 h Induction of autophagy in human U87MG cells by MDC staining at 43.4 uM after 4 hrs 17261067
MDA-MB-231 cells Function assay 2.5-5.8 μM 24 h Induction of apoptosis in human MDA-MB-231 cells assessed as increase in expression of cleaved PARP at 2.5 to 5.8 uM after 24 hrs by SDS-polyactylamide gel electrophoresis 25453798
SF188 cells Function assay 108.4 μM 4 h Induction of autophagy in human SF188 cells by MDC staining at 108.4 uM after 4 hrs 17261067
SF188 cells Function assay 43.4 μM 4 h Induction of autophagy in human SF188 cells by MDC staining at 43.4 uM after 4 hrs 17261067
SF188 cells Function assay 108.4 μM Activation of caspase 3 in human SF188 cells at 108.4 uM 17261067
SF188 cells Function assay 108.4 μM Activation of caspase 7 in human SF188 cells at 108.4 uM 17261067
SF188 cells Cytotoxicity assay 24 h Cytotoxicity against human SF188 cells after 24 hrs 17261067
U87MG cells Cytotoxicity assay 24 h Cytotoxicity against human U87MG cells after 24 hrs 17261067
U87MG cells Function assay 2 h Increase in superoxide anion level in human U87MG cells after 2 hrs 17261067
U87 cell Cytotoxicity assay 72 h Cytotoxicity against human U87 cells after 72 hrs by MTT assay 22342101
Hep3B cells Cytotoxicity assay 72 h Cytotoxicity against human Hep3B cells after 72 hrs by MTT assay 22342101
LLC-PK1 Cytotoxicity assay 4 h Cytotoxicity against pig LLC-PK1 cells after 4 hrs by MTT assay 25453798
MDA-MB-231 cells Proliferation assay 4 h Antiproliferative activity against human MDA-MB-231 cells after 4 hrs by MTT assay 25453798
COLO205 cells Cytotoxicity assay 72 h Cytotoxicity against human COLO205 cells after 72 hrs by MTT assay 21216145
HL60 cells Cytotoxicity assay 72 h Cytotoxicity against human HL60 cells after 72 hrs by MTT assay 21216145
HepG2 cells Cytotoxicity assay 72 h Cytotoxicity against human HepG2 cells after 72 hrs by MTT assay 21216145
MCF7 cells Cytotoxicity assay 72 h Cytotoxicity against human MCF7 cells after 72 hrs by MTT assay 21216145
SF188 cells Function assay 2 h Increase in superoxide anion level in human SF188 cells after 2 hrs 17261067
HepG2 cells Cytotoxicity assay 48 h Cytotoxicity against human HepG2 cells after 48 hrs by MTT assay 26420067
HL60 cells Cytotoxicity assay 48 h Cytotoxicity against human HL60 cells after 48 hrs by MTT assay 26420067
他の多くの細胞株試験データをご覧になる場合はこちらをクリックして下さい

生物活性

製品説明 20(S)-Protopanaxadiol (PPD, 20-Epiprotopanaxadiol), the main intestinal metabolite of ginsenosides, is one of the active ingredients in ginseng. 20(S)-Protopanaxadiol inhibits P-glycoprotein in multidrug resistant cancer cells.
In Vitro
In vitro

Concentrations causing growth inhibition of 50% of cells (LC50) for the compounds PPD is 23 μg/ml, for Int-407 cells. The the LC50 for PPD is determined to be 24 μg/ml in Caco-2 cells. In the Int-407 cell line, PPD produces great percentage of sub-G1 (apoptotic) cells(The Int-407 cell line is a human embryonic nonmalignant cell line originating from fetal tissue of approximately 2 months gestation, whereas Caco-2 is a human colon adenocarcinoma cell line). This response is attributed to differences in individual ginsenoside glycosylation and, thus, the hydrophobic-hydrophilic balance [2]. Incubation with PPD also significantly reduces the viability of U251-MG and U87-MG cells in a dose- and time-dependent manner. The cytotoxic effect of PPD is accompanied by reduced expression of cell adhesion proteins, including N-cadherin and integrin β1, which leads to reduced phosphorylation of focal adhesion kinase. Furthermore, incubation with PPD reduces the expression of cyclin D1 and subsequently induces cell-cycle arrest at the G1 phase[3].

細胞実験 細胞株 Int-407 and Caco-2 cell lines
濃度 23 μg/ml(Int-407 cells); 24 µg/mL(Caco-2 cells)
反応時間 24, 48, 72 h
実験の流れ

Int-407 and Caco-2 cells are seeded at a concentration of 1×106 cells/mL in 24-well plates in separate experiments. Test ginsenosides (PPD, PPT, Rh2) are added to wells to the LC50 concentration determined previously from the MTT assays. The ginsenoside concentrations used for Int-407 cells are 23, 26, and 53 µg/mL, respectively, for PPD, PPT, and Rh2. In a similar experiment using Caco-2 cells, 24 µg/mL for both PPD and PPT and 55 µg/mL for Rh2 is used. Untreated cells represented the control. Cells are incubated at 37℃ in a 5% CO2 humidified incubator for 24, 48, and 72 h. Cell-free supernatant are obtained by centrifugation(400g) for 10 min, and the lactate dehydrogenase (LDH) assay is conducted as previously reported.

In Vivo
In Vivo

Treatment with PPD(20(S)-Protopanaxadiol) and PPT(20(S)-Protopanaxatriol) prior to immobilization stress increase the time spent in open arms and open arm entries in the elevated plus-maze (EPM) test. Treatment with PPD potently suppresses immobilization stress-induced serum levels of corticosterone and interleukin (IL)-6 by the enzyme-linked immunosorbent assay. PPD and PPT may exhibit the anxiolytic effect via γ-aminobutyrateA (GABAA) receptor(s) and serotonergic receptor(s), respectively, and PPD may have an anti-inflammatory effect that is more potent than that of PPT[1].

動物実験 動物モデル Male ICR mice
投与量 5 or 10 mg/kg
投与経路 p.o.

化学情報

分子量 460.73 化学式

C30H52O3

CAS No. 30636-90-9 SDF Download (20S)-Protopanaxadiol SDFをダウンロードする
Smiles CC(=CCCC(C)(C1CCC2(C1C(CC3C2(CCC4C3(CCC(C4(C)C)O)C)C)O)C)O)C
保管

In vitro
Batch:

DMSO : 92 mg/mL ( (199.68 mM); 吸湿したDMSOは溶解度を減少させます。新しいDMSOをご使用ください。)

Ethanol : 33 mg/mL

Water : Insoluble

モル濃度計算器

in vivo
Batch:

Add solvents to the product individually and in order.

投与溶液組成計算機

実験計算

モル濃度計算器

質量 濃度 体積 分子量

投与溶液組成計算機(クリア溶液)

ステップ1:実験データを入力してください。(実験操作によるロスを考慮し、動物数を1匹分多くして計算・調製することを推奨します)

mg/kg g μL

ステップ2:投与溶媒の組成を入力してください。(ロット毎に適した溶解組成が異なる場合があります。詳細については弊社までお問い合わせください)

% DMSO % % Tween 80 % ddH2O
%DMSO %

計算結果:

投与溶媒濃度: mg/ml;

DMSOストック溶液調製方法: mg 試薬を μL DMSOに溶解する(濃度 mg/mL, 注:濃度が当該ロットのDMSO溶解度を超える場合はご連絡ください。 )

投与溶媒調製方法:Take μL DMSOストック溶液に μL PEG300,を加え、完全溶解後μL Tween 80,を加えて完全溶解させた後 μL ddH2O,を加え完全に溶解させます。

投与溶媒調製方法:μL DMSOストック溶液に μL Corn oil,を加え、完全溶解。

注意:1.ストック溶液に沈殿、混濁などがないことをご確認ください;
2.順番通りに溶剤を加えてください。次のステップに進む前に溶液に沈殿、混濁などがないことを確認してから加えてください。ボルテックス、ソニケーション、水浴加熱など物理的な方法で溶解を早めることは可能です。

技術サポート

ストックの作り方、阻害剤の保管方法、細胞実験や動物実験の際に注意すべき点など、製品を取扱う時に問い合わせが多かった質問に対しては取扱説明書でお答えしています。

Handling Instructions

他に質問がある場合は、お気軽にお問い合わせください。

* 必須

大学・企業名を記入してください
名前を記入してください
電子メール・アドレスを記入してください 有効なメールアドレスを入力してください
お問い合わせ内容をご入力ください
Tags: (20S)-Protopanaxadiolを買う | (20S)-Protopanaxadiol ic50 | (20S)-Protopanaxadiol供給者 | (20S)-Protopanaxadiolを購入する | (20S)-Protopanaxadiol費用 | (20S)-Protopanaxadiol生産者 | オーダー(20S)-Protopanaxadiol | (20S)-Protopanaxadiol化学構造 | (20S)-Protopanaxadiol分子量 | (20S)-Protopanaxadiol代理店