Oligomycin A

Oligomycin A (MCH 32), a dominant analog of the isomers, is an inhibitor of mitochondrial F1FO ATP synthase which inhibits oxidative phosphorylation and all the ATP-dependent processes occurring on the coupling membrane of mitochondria. It inhibits ATP synthase by blocking its proton channel (Fo subunit), which is necessary for oxidative phosphorylation of ADP to ATP. It also induces apoptosis in a variety of cell types.

CAS No. 579-13-5

サイズ	価格(税別)	在庫状況
10mM (1mL in DMSO)	JPY 38500	国内在庫なし(納期7~10日)
5mg	JPY 29500	国内在庫あり
25mg	JPY 88500	国内在庫あり

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製品安全説明書

現在のバッチを見る：純度： 99.98%

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Oligomycin Aと併用されることが多い化合物

FCCP

Oligomycin A inhibits ATP synthase and reduces OCR, whereas FCCP uncouples oxygen consumption from ATP production and raises OCR to a maximal value in SK-Mel-28 cells.

Plitzko B, et al. Bio Protoc. 2018 May 20;8(10):e2850.

Trastuzumab (anti-HER2)

Oligomycin A and Trastuzumab combination treatment leads to regression of trastuzumab-resistant tumors in vivo.

Gale M, et al. Cancer Res. 2020 Feb 1;80(3):524-535.

Tomatidine Hydrochloride

Oligomycin A and Tomatidine sensitizes Staphylococcus aureus to polymyxin B.

Vestergaard M, et al. Antibiotics (Basel). 2021 Apr 2;10(4):376.

Imatinib

Oligomycin-A and Imatinib combined treatment synergistically reduces colony formation in K562 cells.

Alvarez-Calderon F, et al. Clin Cancer Res. 2015 Mar 15;21(6):1360-72.

Oligomycin A関連製品

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関連化合物ライブラリー	FDA-approved Drug Library Natural Product Library Ion Channel Ligand Library Exosome Secretion Related Compound Library Calcium Channel Blocker Library	もっと見る

Antineoplastic and Immunosuppressive Antibiotics阻害剤の選択性比較

Cell Data

Cell Lines	Assay Type	Concentration	活性情報	PMID
Vero cells	Function assay	0.01, 0.1, 1, and 10 μM	a decrease in the viability of Vero cells (~20% reduction) at increasing concentrations.	26859745
HepG2 cells	Function assay	25, 50, 75, 100 μM	elevated the glucose uptake level but not dose-dependent	30205572
ISE6 cells	Function assay	0.01, 0.1, 1, and 10 μM	a decrease in the viability of ISE6 cells (~60%) at increasing concentrations.	26859745
他の多くの細胞株試験データをご覧になる場合はこちらをクリックして下さい

生物活性

製品説明

Oligomycin A (MCH 32), a dominant analog of the isomers, is an inhibitor of mitochondrial F1FO ATP synthase which inhibits oxidative phosphorylation and all the ATP-dependent processes occurring on the coupling membrane of mitochondria. It inhibits ATP synthase by blocking its proton channel (Fo subunit), which is necessary for oxidative phosphorylation of ADP to ATP. It also induces apoptosis in a variety of cell types.

Targets

ATP synthase ^[1]
(Cell-free assay)

In Vitro
In vitro	Oligomycin A is an inhibitor of ATP synthase, inhibits oxidative phosphorylation and all the ATP-dependent processes occurring on the coupling membrane of mitochondria.Oligomycin A inhibits ATP synthase by blocking its proton channel (Fo subunit), which is necessary for oxidative phosphorylation of ADP to ATP. The inhibition of ATP synthesis by oligomycin A will significantly reduce electron flow through the electron transport chain; however, electron flow is not stopped completely due to a process known as proton leak or mitochondrial uncoupling. ^[1] In a group of cancer cells, Oligomycin at 100 ng/ml completely inhibits oxidative phosphorylation activity in 1 h and induces various levels of glycolysis gains by 6 h. ^[2] Oligomycin, inhibitor of the F0 part of H⁺-ATP-synthase, suppresses the TNF-induced apoptosis. ^[3] Oligomycin inhibits mitochondrial respiration in melanoma, sensitizes melanoma cells to therapy, blocks the emergence of the slow-cycling, long-term tumor-maintaining melanoma cells. ^[4]
細胞実験	細胞株	H1229
	濃度	100 ng/ml
	反応時間	1 h
	実験の流れ	ATP and Oligomycin Dose-Response Growth Measurement. Cellular ATP changes are measured by CellTiter-Glo reagent. To measure oligomycin dose-response curves, the cells are plated in 96-well plates at about 400–500 cells/well in 100 μl of culture, dosed the next day, and grown for 4 additional days followed by assaying with Cell-Titer-Glo reagent. The dose-response curves are plotted with nonlinear regression analysis of GraphPad Prism.
実験結果図	Methods	Biomarkers	結果図	PMID
実験結果図	Western blot	p-GCN2 / GCN2 / p-eIF2α / eIF2α / ATF4 / xCT pAkt / Her2 / Raf / Hsp70 / Hsp90 p-T389 S6K1 / S6K1 / p-T172 AMPK / AMPK Cox1p / Cox2p / Cox3p / Cox4p		27708226

In Vivo
In Vivo	Oligomycin A (MCH 32) is an inhibitor of mitochondrial F0F1-ATPase.
動物実験	動物モデル	Male Sprague-Dawley rats
	投与量	0.5 mg/kg
	投与経路	i.v.

参考
[1] Jastroch M, et al. Essays Biochem. 2010;47:53-67. [2] Hao W, et al. J Biol Chem, 2010, 285(17), 12647-12654. [3] Shchepina LA, et al. Oncogene, 2002, 21(53):8149-8157. [4] Roesch A, et al. Cancer Cell, 2013, 23(6), 811-825. [5] Hu Q, et al. Stroke. 2017 Jun;48(6):1655-1664. [6] Shin YK, et al. Cancer Res. 2005 Apr 15;65(8):3162-70. + 展開

参考

+ 展開

化学情報

分子量	791.06	化学式	C₄₅H₇₄O₁₁
CAS No.	579-13-5	SDF	Download Oligomycin A SDFをダウンロードする
Smiles	CCC1CCC2C(C(C(C3(O2)CCC(C(O3)CC(C)O)C)C)OC(=O)C=CC(C(C(C(=O)C(C(C(C(=O)C(C(C(CC=CC=C1)C)O)(C)O)C)O)C)C)O)C)C
保管	3年-20°C粉	溶液の保管冷凍と解凍の繰り返しを避けるため小分けにしてください	1年-80°Cin solvent
保管	3年-20°C粉	溶液の保管冷凍と解凍の繰り返しを避けるため小分けにしてください	1个月-20°Cin solvent

In vitro
Batch:

DMSO : 100 mg/mL ( (126.41 mM)；吸湿したDMSOは溶解度を減少させます。新しいDMSOをご使用ください。)

Water : Insoluble

Ethanol : Insoluble

モル濃度計算器

in vivo
Batch:

Add solvents to the product individually and in order.

投与溶液組成計算機

実験計算

モル濃度計算器

質量	濃度	体積	分子量
=	×	×

希釈計算器分子量計算器

投与溶液組成計算機(クリア溶液)

ステップ１：実験データを入力してください。（実験操作によるロスを考慮し、動物数を1匹分多くして計算・調製することを推奨します）

投与量 mg/kg 動物平均体重 g 投与体積（動物毎）μL 動物数匹

ステップ２：投与溶媒の組成を入力してください。（ロット毎に適した溶解組成が異なる場合があります。詳細については弊社までお問い合わせください）

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

計算結果：

投与溶媒濃度： mg/ml;

DMSOストック溶液調製方法： mg 試薬を μL DMSOに溶解する（濃度 mg/mL, 注：濃度が当該ロットのDMSO溶解度を超える場合はご連絡ください。 )

投与溶媒調製方法：Take μL DMSOストック溶液に μL PEG300，を加え、完全溶解後μL Tween 80，を加えて完全溶解させた後 μL ddH₂O，を加え完全に溶解させます。

投与溶媒調製方法：μL DMSOストック溶液に μL Corn oil，を加え、完全溶解。

注意：１.ストック溶液に沈殿、混濁などがないことをご確認ください；
２.順番通りに溶剤を加えてください。次のステップに進む前に溶液に沈殿、混濁などがないことを確認してから加えてください。ボルテックス、ソニケーション、水浴加熱など物理的な方法で溶解を早めることは可能です。

C1=C0/X	C1:	LOG(C1):
C2=C1/X	C2:	LOG(C2):
C3=C2/X	C3:	LOG(C3):
C4=C3/X	C4:	LOG(C4):
C5=C4/X	C5:	LOG(C5):
C6=C5/X	C6:	LOG(C6):
C7=C6/X	C7:	LOG(C7):
C8=C7/X	C8:	LOG(C8):