Oridonin

別名:NSC-250682, Isodonol, Rubescenin

Oridonin, a diterpenoid purified from Rabdosia rubescens, is a traditional agent with antitumor, anti-bacterial and anti-inflammatory effects. Oridonin inhibits AKT1 and AKT2 kinase activity with IC50 of 8.4 μM and 8.9 μM, respectively.

Oridonin化学構造

CAS No. 28957-04-2

サイズ 価格(税別) 在庫状況
10mM (1mL in DMSO) JPY 16900 国内在庫あり
JPY 13600 国内在庫あり

代表番号: 045-509-1970|電子メール:[email protected]
よく尋ねられる質問

文献中Selleckの製品使用例(22)

カスタマーフィードバック1个实验数据

製品安全説明書

現在のバッチを見る: 純度: 99.99%
99.99

Oridonin関連製品

シグナル伝達経路

Akt阻害剤の選択性比較

Cell Data

Cell Lines Assay Type Concentration Incubation Time 活性情報 PMID
HCT116 Cell cycle arrest assay 3 uM 24 hrs Induction in cell cycle arrest in human HCT116 cells assessed as inhibition in cyclin A protein expression level at 3 uM incubated for 24 hrs by Western blot analysis 30981113
HCT116 Cell cycle arrest assay 3 uM 24 hrs Induction in cell cycle arrest in human HCT116 cells assessed as inhibition in cyclin B1 protein expression level at 3 uM incubated for 24 hrs by Western blot analysis 30981113
HCT116 Apoptotic assay 1 uM 24 hrs Induction of apoptotic morphological change in human HCT116 cells assessed as loss of original morphology at 1 uM incubated for 24 hrs by haematoxylin-eosin staining based microscopic method 30981113
HCT116 Apoptotic assay 1 uM 24 hrs Induction of apoptotic morphological change in human HCT116 cells assessed as formation of unstructured state at 1 uM incubated for 24 hrs by haematoxylin-eosin staining based microscopic method 30981113
HCT116 Apoptotic assay 1 uM 24 hrs Induction of apoptotic morphological change in human HCT116 cells assessed as formation of volume swelling at 1 uM incubated for 24 hrs by haematoxylin-eosin staining based microscopic method 30981113
HCT116 Apoptotic assay 1 uM 24 hrs Induction of apoptotic morphological change in human HCT116 cells assessed as formation of condensed nuclei at 1 uM incubated for 24 hrs by haematoxylin-eosin staining based microscopic method 30981113
HCT116 Apoptotic assay 1 uM 24 hrs Induction of apoptotic morphological change in human HCT116 cells assessed as cell lysis at 1 uM incubated for 24 hrs by haematoxylin-eosin staining based microscopic method 30981113
HCT116 Apoptotic assay 1 uM 24 hrs Induction of apoptotic morphological change in human HCT116 cells assessed as membrane shrinkage at 1 uM incubated for 24 hrs by haematoxylin-eosin staining based microscopic method 30981113
HepG2 Function assay 0.1 to 1 uM 10 days Inhibition of colony formation in human HepG2 cells at 0.1 to 1 uM incubated for 10 days by crystal violet staining based microscopic method 31200238
PLC/PRF/5 Function assay 0.001 to 1 uM 10 days Inhibition of colony formation in human PLC/PRF/5 cells assessed as decrease in colony size at 0.001 to 1 uM incubated for 10 days by crystal violet staining based microscopic method 31200238
HL60 Cytotoxicity assay 48 hrs Cytotoxicity against human HL60 cells after 48 hrs by MTT assay, IC50=2.5μM 23819871
RAW264.7 Antiinflammatory assay 18 hrs Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production after 18 hrs by Griess method, IC50=5.2μM 23819871
K562 Antiproliferative assay 72 hrs Antiproliferative activity against human K562 cells after 72 hrs by MTT assay, IC50=4.33μM 28165738
Bel7402 Antiproliferative assay 72 hrs Antiproliferative activity against human Bel7402 cells after 72 hrs by MTT assay, IC50=5.41μM 28165738
MGC803 Antiproliferative assay 72 hrs Antiproliferative activity against human MGC803 cells after 72 hrs by MTT assay, IC50=9.06μM 28165738
KB/CP4 Antiproliferative assay 48 hrs Antiproliferative activity against human KB/CP4 cells after 48 hrs by MTT assay, IC50=10.8μM 28165738
KB-3-1 Antiproliferative assay 48 hrs Antiproliferative activity against human KB-3-1 cells after 48 hrs by MTT assay, IC50=13.24μM 28165738
BEL7404/CP20 Antiproliferative assay 48 hrs Antiproliferative activity against human BEL7404/CP20 cells after 48 hrs by MTT assay, IC50=17.17μM 28165738
MCF7 Antiproliferative assay 72 hrs Antiproliferative activity against human MCF7 cells after 72 hrs by MTT assay, IC50=17.89μM 28165738
Bel7404 Antiproliferative assay 48 hrs Antiproliferative activity against human Bel7404 cells after 48 hrs by MTT assay, IC50=17.92μM 28165738
NCI-H460 Antiproliferative assay 48 hrs Antiproliferative activity against human NCI-H460 cells after 48 hrs by MTT assay, IC50=20.51μM 28165738
NCI-H460/MX20 Antiproliferative assay 48 hrs Antiproliferative activity against human NCI-H460/MX20 cells after 48 hrs by MTT assay, IC50=25.86μM 28165738
K562 Antiproliferative assay 72 hrs Antiproliferative activity against human K562 cells assessed as reduction in cell viability after 72 hrs by MTT assay, IC50=4.64μM 30921757
SGC7901 Antiproliferative assay 72 hrs Antiproliferative activity against human SGC7901 cells assessed as reduction in cell viability after 72 hrs by MTT assay, IC50=7.13μM 30921757
Bel7402 Antiproliferative assay 72 hrs Antiproliferative activity against human Bel7402 cells assessed as reduction in cell viability after 72 hrs by MTT assay, IC50=7.85μM 30921757
HL60 Antiproliferative assay 72 hrs Antiproliferative activity against human HL60 cells assessed as reduction in cell viability after 72 hrs by MTT assay, IC50=9.46μM 30921757
PC3 Antiproliferative assay 72 hrs Antiproliferative activity against human PC3 cells assessed as reduction in cell viability after 72 hrs by MTT assay, IC50=10.07μM 30921757
A549 Antiproliferative assay 72 hrs Antiproliferative activity against human A549 cells assessed as reduction in cell viability after 72 hrs by MTT assay, IC50=18.15μM 30921757
L02 Cytotoxicity assay 72 hrs Cytotoxicity against human L02 cells assessed as reduction in cell viability after 72 hrs by MTT assay, IC50=19.53μM 30921757
HCT116 Antiproliferative assay 48 hrs Antiproliferative activity against human HCT116 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay, IC50=6.84μM 30981113
L02 Cytotoxicity assay 48 hrs Cytotoxicity in human L02 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay, IC50=6.97μM 30981113
Bel7402 Antiproliferative assay 48 hrs Antiproliferative activity against human Bel7402 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay, IC50=9.59μM 30981113
MCF7 Antiproliferative assay 48 hrs Antiproliferative activity against human MCF7 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay, IC50=17.56μM 30981113
MDA-MB-231 Antiproliferative assay 48 hrs Antiproliferative activity against human MDA-MB-231 cells incubated for 48 hrs by MTT assay, EC50=29.4μM 30981113
PLC/PRF/5 Antiproliferative assay 72 hrs Antiproliferative activity against human PLC/PRF/5 cells incubated for 72 hrs by CCK8 cells, IC50=7.41μM 31200238
HepG2 Antiproliferative assay 72 hrs Antiproliferative activity against human HepG2 cells incubated for 72 hrs by CCK8 cells, IC50=8.12μM 31200238
RPMI8226 Antiproliferative assay 72 hrs Antiproliferative activity against human RPMI8226 cells incubated for 72 hrs by CCK8 cells, IC50=9.25μM 31200238
LO2 Cytotoxicity assay 72 hrs Cytotoxicity against human LO2 cells incubated for 72 hrs by CCK8 cells, IC50=17.47μM 31200238
A549 Antiproliferative assay 72 hrs Antiproliferative activity against human A549 cells incubated for 72 hrs by CCK8 cells, IC50=30.4μM 31200238
K562 Antiproliferative assay 72 hrs Antiproliferative activity against human K562 cells assessed as reduction in cell viability after 72 hrs by MTT assay, IC50=4.79μM 31202992
SGC7901 Antiproliferative assay 72 hrs Antiproliferative activity against human SGC7901 cells assessed as reduction in cell viability after 72 hrs by MTT assay, IC50=7.87μM 31202992
Bel7402 Antiproliferative assay 72 hrs Antiproliferative activity against human Bel7402 cells assessed as reduction in cell viability after 72 hrs by MTT assay, IC50=8.31μM 31202992
A549 Antiproliferative assay 72 hrs Antiproliferative activity against human A549 cells assessed as reduction in cell viability after 72 hrs by MTT assay, IC50=17.8μM 31202992
L02 Cytotoxicity assay 72 hrs Cytotoxicity against human L02 cells assessed as reduction in cell viability after 72 hrs by MTT assay, IC50=18.68μM 31202992
TE1 Antiproliferative assay 72 hrs Antiproliferative activity against human TE1 cells assessed as reduction in cell viability incubated for 72 hrs by SRB assay, IC50=12.73μM 31494472
A549 Antiproliferative assay 72 hrs Antiproliferative activity against human A549 cells assessed as reduction in cell viability incubated for 72 hrs by SRB assay, IC50=13.45μM 31494472
MGC803 Antiproliferative assay 72 hrs Antiproliferative activity against human MGC803 cells assessed as reduction in cell viability incubated for 72 hrs by SRB assay, IC50=14.13μM 31494472
MCF7 Antiproliferative assay 72 hrs Antiproliferative activity against human MCF7 cells assessed as reduction in cell viability incubated for 72 hrs by SRB assay, IC50=21.97μM 31494472
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生物活性

製品説明 Oridonin, a diterpenoid purified from Rabdosia rubescens, is a traditional agent with antitumor, anti-bacterial and anti-inflammatory effects. Oridonin inhibits AKT1 and AKT2 kinase activity with IC50 of 8.4 μM and 8.9 μM, respectively.
Targets
Akt1 [5]
(Cell-free assay)
Akt2 [5]
(Cell-free assay)
8.4 μM 8.9 μM
In Vitro
In vitro

Oridonin effectively inhibites the proliferation of a wide variety of cancer cells including those from prostate (LNCaP, DU145, PC3), breast (MCF-7, MDA-MB231), non-small cell lung (NSCL) (NCI-H520, NCI-H460, NCI-H1299) cancers, acute promyelocytic leukemia (NB4), and glioblastoma multiforme (U118, U138) with ED50s ranging from 1.8 to 7.5 μg/ml. [1] The antitumor activities of Oridonin may due to its inhibitory effects on NF-kappaB and MAPKs signal pathways. [2] [3]

実験結果図 Methods Biomarkers 結果図 PMID
Western blot BCR-ABL / Myc p-AMPK / AMPK / Glut1 / LC3 28128329
Immunofluorescence HSF1 p65 28128329
Growth inhibition assay Cell viability 26496199
In Vivo
In Vivo

LD50: Mice 35-40mg/kg. [4]

化学情報

分子量 364.43 化学式

C20H28O6

CAS No. 28957-04-2 SDF Download Oridonin SDFをダウンロードする
保管

In vitro
Batch:

DMSO : 72 mg/mL ( (197.56 mM); 吸湿したDMSOは溶解度を減少させます。新しいDMSOをご使用ください。)

Ethanol : 34 mg/mL

Water : Insoluble

モル濃度計算器

in vivo
Batch:

Add solvents to the product individually and in order.

投与溶液組成計算機

実験計算

モル濃度計算器

質量 濃度 体積 分子量

投与溶液組成計算機(クリア溶液)

ステップ1:実験データを入力してください。(実験操作によるロスを考慮し、動物数を1匹分多くして計算・調製することを推奨します)

mg/kg g μL

ステップ2:投与溶媒の組成を入力してください。(ロット毎に適した溶解組成が異なる場合があります。詳細については弊社までお問い合わせください)

% DMSO % % Tween 80 % ddH2O
%DMSO %

計算結果:

投与溶媒濃度: mg/ml;

DMSOストック溶液調製方法: mg 試薬を μL DMSOに溶解する(濃度 mg/mL, 注:濃度が当該ロットのDMSO溶解度を超える場合はご連絡ください。 )

投与溶媒調製方法:Take μL DMSOストック溶液に μL PEG300,を加え、完全溶解後μL Tween 80,を加えて完全溶解させた後 μL ddH2O,を加え完全に溶解させます。

投与溶媒調製方法:μL DMSOストック溶液に μL Corn oil,を加え、完全溶解。

注意:1.ストック溶液に沈殿、混濁などがないことをご確認ください;
2.順番通りに溶剤を加えてください。次のステップに進む前に溶液に沈殿、混濁などがないことを確認してから加えてください。ボルテックス、ソニケーション、水浴加熱など物理的な方法で溶解を早めることは可能です。

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Tags: Oridoninを買う | Oridonin ic50 | Oridonin供給者 | Oridoninを購入する | Oridonin費用 | Oridonin生産者 | オーダーOridonin | Oridonin化学構造 | Oridonin分子量 | Oridonin代理店