Puromycin 2HCl

別名:CL13900 2HCl

Puromycin 2HCl is an aminonucleoside antibiotic derived from Streptomyces alboniger. It is an inhibitor of protein synthesis and has been used to study transcription regulatory mechanisms that control the sequential and coordinated expression of genes during cell differentiation.

Puromycin 2HCl化学構造

CAS No. 58-58-2

サイズ 価格(税別) 在庫状況
10mM (1mL in DMSO) JPY 29500 国内在庫あり
JPY 22000 国内在庫あり
JPY 37000 国内在庫あり
JPY 55500 国内在庫あり
JPY 145500 国内在庫なし(納期7~10日)

代表番号: 045-509-1970|電子メール:[email protected]
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製品安全説明書

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Puromycin 2HClと併用されることが多い化合物

Blasticidin S HCl


Puromycin and Blasticidin S inhibits the proliferation and cell viability of metastatic castration-resistant prostate cancer (mCRPC) cells PC3/DU145.

Oseni SO, et al. bioRxiv (2021): 2021-08.

Melatonin


Melatonin potentiates puromycin-induced apoptosis with caspase-3 and AMPK activation in HL-60 cells.

Koh W, et al. J Pineal Res. 2011 May;50(4):367-73.

Bortezomib


Puromycin and Bortezomib combination significantly increases the antitumor activity of bortezomib in a mouse model of multiple myeloma.

Neznanov N, et al. Oncotarget. 2011 Mar; 2(3): 209–221.

Glycocholic acid


Puromycin and Glycocholate combination results in a higher absorption enhancement of leucine enkephalin (Leu-Enk).

[1] Agu RU, et al. Int J Pharm. 2002 Apr 26;237(1-2):179-91.

Doxorubicin


Puromycin and Doxorubicin combination promotes apoptosis by attenuating c-Myc and Bcl-2 and activating p53 in p53 wild-type HCT116 cells.

Jung JH, et al. Cancers (Basel). 2019 Apr 24;11(4):582.

Puromycin 2HCl関連製品

Antineoplastic and Immunosuppressive Antibiotics阻害剤の選択性比較

Cell Data

Cell Lines Assay Type Concentration Incubation Time 活性情報 PMID
HepG2 Growth inhibition assay 72 h Growth inhibition of human HepG2 cells after 72 hrs by SRB assay, IC50=0.21 μM. 18512984
K562 Growth inhibition assay 72 h Growth inhibition of human K562 cells after 72 hrs by SRB assay, IC50=0.22 μM. 18313307
HepG2 Growth inhibition assay 72 h Growth inhibition of human HepG2 cells after 72 hrs by MTT assay, IC50=0.23 μM. 18313307
K562 Growth inhibition assay 72 h Growth inhibition of doxorubicin-resistant human K562 cells after 72 hrs by SRB assay, IC50=26.83 μM. 18313307
HEK239 Cytotoxicity assay 72 h Cytotoxicity against HEK239 cells after 72 hrs by Alamar blue assay, IC50=0.42 μM. 20558060
KB3-1 Growth inhibition assay 72 hrs Growth inhibition of human KB3-1 cells after 72 hrs by MTT assay, IC50 = 0.21 μM. 18313307
HepG2/Dox Growth inhibition assay 72 hrs Growth inhibition of multidrug resistant human HepG2/Dox cells after 72 hrs by SRB assay, IC50 = 20.2 μM. 18512984
HEK293 Cytotoxicity assay 72 hrs Cytotoxicity against HEK293 cells assessed as cell viability after 72 hrs by resazurin-based plate reader analysis, IC50 = 0.361 μM. 26651537
HEK293 Cytotoxicity assay 72 hrs Cytotoxicity against HEK293 cells assessed as growth inhibition after 72 hrs by alamar blue viability assay, IC50 = 0.3505 μM. 27060763
HEK293 Growth inhibition assay 72 hrs Growth inhibition of HEK293 cells after 72 hrs by PrestoBlue staining based fluorescence assay, IC50 = 0.36 μM. 28001067
SU-DHL10 Cytotoxicity assay 72 hrs Cytotoxicity against human SU-DHL10 cells assessed as growth inhibition after 72 hrs by CellTitre-Glo luminescent assay, CC50 = 0.11 μM. 28523103
MIAPaCa2 Cytotoxicity assay 72 hrs Cytotoxicity against human MIAPaCa2 cells assessed as growth inhibition after 72 hrs by CellTitre-Glo luminescent assay, CC50 = 0.2 μM. 28523103
MDA-MB-231 Cytotoxicity assay 72 hrs Cytotoxicity against human MDA-MB-231 cells assessed as growth inhibition after 72 hrs by CellTitre-Glo luminescent assay, CC50 = 0.48 μM. 28523103
HEK293 Cytotoxicity assay 72 hrs Cytotoxicity against HEK293 cells assessed as cell growth inhibition after 72 hrs by Alamar blue assay, IC50 = 0.46 μM. 28774427
HL60 Antiparasitic assay 72 hrs Antiproliferative activity against human HL60 cells after 72 hrs by MTT assay, EC50 = 0.055 μM. 28803047
MOLT4 Antiparasitic assay 72 hrs Antiproliferative activity against human MOLT4 cells after 72 hrs by MTT assay, EC50 = 0.17 μM. 28803047
Vero Cytotoxicity assay 72 hrs Cytotoxicity against African green monkey Vero cells assessed as reduction in cell proliferation after 72 hrs by MTT assay, CC50 = 2.8 μM. 28803047
HEK293 Cytotoxicity assay 72 hrs Cytotoxicity against HEK293 cells after 72 hrs by resazurin dye based assay, IC50 = 0.45 μM. 29236492
HEK293T Cytotoxicity assay 72 hrs Cytotoxicity against human HEK293T cells assessed as decrease in cell viability after 72 hrs by CellTiter-Glo reagent based luminescence assay, CC50 = 0.42 μM. 29469575
HCT8 Antiparasitic assay 48 hrs Antiparasitic activity against Cryptosporidium parvum BGF infected in human HCT8 cells incubated for 48 hrs by FITC/DAPI staining based fluorescence assay, EC50 = 0.55 μM. 29469575
HCT8 Antiparasitic assay 48 hrs Antiparasitic activity against Cryptosporidium parvum SPL infected in human HCT8 cells incubated for 48 hrs by FITC/DAPI staining based fluorescence assay, EC50 = 0.6 μM. 29469575
HepG2 Cytotoxicity assay 72 hrs Cytotoxicity against human HepG2 cells assessed as decrease in cell viability after 72 hrs by CellTiter-Glo reagent based luminescence assay, CC50 = 0.83 μM. 29469575
HEK293 Cytotoxicity assay 72 hrs Cytotoxicity against HEK293 cells assessed as inhibition of cell growth after 72 hrs by alamar blue assay, IC50 = 0.46 μM. 29533611
HEK293 Cytotoxicity assay 72 hrs Cytotoxicity against human HEK293 cells after 72 hrs by resazurin dye based fluorescence assay, IC50 = 0.4 μM. 29969262
A549 Cytotoxicity assay 48 hrs Cytotoxicity against human A549 cells assessed as decrease in cell viability after 48 hrs, IC50 = 0.3 μM. 30418763
PC3 Cytotoxicity assay 48 hrs Cytotoxicity against human PC3 cells assessed as decrease in cell viability after 48 hrs, IC50 = 0.3 μM. 30418763
Huh7 Cytotoxicity assay NOVARTIS: Cytotoxicity against human hepatocellular carcinoma cell line (Huh7), CC50=6.63 μM. 18579783
J774A1 Cytotoxicity assay Cytotoxicity against mouse J774A1 cells by trypan blue exclusion assay, IC50=10 μM. 22934636
A549 Cytotoxicity assay Cytotoxicity against human A549 cells, IC50 = 0.6 μM. 27089214
PC3 Cytotoxicity assay Cytotoxicity against human PC3 cells, IC50 = 0.7 μM. 27089214
HEK293 Cytotoxicity assay Cytotoxicity against HEK293 cells by alamar blue assay, IC50 = 0.00035 μM. 27212070
HEK293 Growth inhibition assay Growth inhibition of HEK293 cells, IC50 = 0.399 μM. 28400231
HCT8 Cytotoxicity assay Cytotoxicity against human HCT8 cells, EC50 = 6.1 μM. 29469575
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生物活性

製品説明 Puromycin 2HCl is an aminonucleoside antibiotic derived from Streptomyces alboniger. It is an inhibitor of protein synthesis and has been used to study transcription regulatory mechanisms that control the sequential and coordinated expression of genes during cell differentiation.
In Vitro
In vitro

The antibiotic puromycin is produced by the actinoniycete, Streptornyces alboniger, and has been used as a tool for studying protein synthesis in a number of systems. [1]

Puromycin can be used for the selection of recombinant cells from noncultured cells. [2]

細胞実験 細胞株 HepG2 cells 
濃度 1.6 μM
反応時間 72 h
実験の流れ

Cells were treated with different concentrations of drug for 72 h.

実験結果図 Methods Biomarkers 結果図 PMID
Western blot Cyclin D1 / CDK4 PARP / Bcl-xL / Bcl-2 / p-Akt / Akt p53 / p21 / c-Myc 31022952
Growth inhibition assay Cell viability 31022952
In Vivo
In Vivo

Puromycin (aminonucleoside) inhibits protein synthesis leading to proteinuria & glomerulosclerosis/nephrosis.[4]

動物実験 動物モデル Male Sprague-Dawley rats
投与量 5 mg/100 g
投与経路 i.v.

化学情報

分子量 544.43 化学式

C22H29N7O5.2HCl

CAS No. 58-58-2 SDF Download Puromycin 2HCl SDFをダウンロードする
Smiles CN(C)C1=NC=NC2=C1N=CN2C3C(C(C(O3)CO)NC(=O)C(CC4=CC=C(C=C4)OC)N)O.Cl.Cl
保管

In vitro
Batch:

DMSO : 100 mg/mL ( (183.67 mM); Warmed with 50℃ water bath; 吸湿したDMSOは溶解度を減少させます。新しいDMSOをご使用ください。)

Water : 100 mg/mL

Ethanol : Insoluble

モル濃度計算器

in vivo
Batch:

Add solvents to the product individually and in order.

投与溶液組成計算機

実験計算

モル濃度計算器

質量 濃度 体積 分子量

投与溶液組成計算機(クリア溶液)

ステップ1:実験データを入力してください。(実験操作によるロスを考慮し、動物数を1匹分多くして計算・調製することを推奨します)

mg/kg g μL

ステップ2:投与溶媒の組成を入力してください。(ロット毎に適した溶解組成が異なる場合があります。詳細については弊社までお問い合わせください)

% DMSO % % Tween 80 % ddH2O
%DMSO %

計算結果:

投与溶媒濃度: mg/ml;

DMSOストック溶液調製方法: mg 試薬を μL DMSOに溶解する(濃度 mg/mL, 注:濃度が当該ロットのDMSO溶解度を超える場合はご連絡ください。 )

投与溶媒調製方法:Take μL DMSOストック溶液に μL PEG300,を加え、完全溶解後μL Tween 80,を加えて完全溶解させた後 μL ddH2O,を加え完全に溶解させます。

投与溶媒調製方法:μL DMSOストック溶液に μL Corn oil,を加え、完全溶解。

注意:1.ストック溶液に沈殿、混濁などがないことをご確認ください;
2.順番通りに溶剤を加えてください。次のステップに進む前に溶液に沈殿、混濁などがないことを確認してから加えてください。ボルテックス、ソニケーション、水浴加熱など物理的な方法で溶解を早めることは可能です。

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